By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- New research on Hydroxybenzoic Acids is the subject of a report. According to news reporting originating in Jackson, Tennessee, by NewsRx journalists, research stated, "Salicylic acid plays a critical role in activating plant defence responses after pathogen attack. Salicylic acid methyltransferase (SAMT) modulates the level of salicylic acid by converting salicylic acid to methyl salicylate."
The news reporters obtained a quote from the research from the United States Department of Agriculture, "Here, we report that a SAMT gene from soybean (GmSAMT1) plays a role in soybean defence against soybean cyst nematode (Heterodera glycines Ichinohe, SCN). GmSAMT1 was identified as a candidate SCN defence-related gene in our previous analysis of soybean defence against SCN using GeneChip microarray experiments. The current study started with the isolation of the full-length cDNAs of GmSAMT1 from a SCN-resistant soybean line and from a SCN-susceptible soybean line. The two cDNAs encode proteins of identical sequences. The GmSAMT1 cDNA was expressed in Escherichia coli. Using in vitro enzyme assays, E.coli-expressed GmSAMT1 was confirmed to function as salicylic acid methyltransferase. The apparent Km value of GmSAMT1 for salicylic acid was approximately 46m. To determine the role of GmSAMT1 in soybean defence against SCN, transgenic hairy roots overexpressing GmSAMT1 were produced and tested for SCN resistance. Overexpression of GmSAMT1 in SCN-susceptible backgrounds significantly reduced the development of SCN, indicating that overexpression of GmSAMT1 in the transgenic hairy root system could confer resistance to SCN."
According to the news reporters, the research concluded: "Overexpression of GmSAMT1 in transgenic hairy roots was also found to affect the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction."
For more information on this research see: Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode. Plant Biotechnology Journal, 2013;11(9):1135-1145. Plant Biotechnology Journal can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Plant Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-7652)
Our news correspondents report that additional information may be obtained by contacting J.Y. Lin, Dept. of Agriculture ARS, Crop Genet Res Unit, Jackson, TN, United States. Additional authors for this research include M. Mazarei, N. Zhao, J.W.J. Zhu, X.F. Zhuang, W.S. Liu, V.R. Pantalone, P.R. Arelli, C.N. Stewart and F. Chen (see also Hydroxybenzoic Acids).
Keywords for this news article include: Cysts, Jackson, Genetics, Tennessee, Nematodes, United States, Salicylic Acids, Carboxylic Acids, Organic Chemicals, Methyltransferases, Hydroxybenzoic Acids, North and Central America, One-Carbon Group Transferases
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