By a News Reporter-Staff News Editor at Gene Therapy Weekly -- A new study on Animal Diseases is now available. According to news reporting out of Chongqing, People's Republic of China, by NewsRx editors, research stated, "Newcastle disease virus (NDV) can cause serious diseases and substantial economic losses to the poultry industry. To gain a better understanding of NDV pathogenesis, several reverse genetics systems for different NDV strains have been established."
Our news journalists obtained a quote from the research from Southwest University, "However, the construction of infectious cDNA clone by conventional restriction digestion/ligation cloning methods is a time-consuming process and has many drawbacks by its nature. To address the problems, we employed a novel and robust ligation-independent cloning (LIC) method for efficient assembly of multiple DNA fragments. Using this method, we successfully generated a NDV minigenome construct within three weeks by assembling RT-PCR products of the VG/GA strain genomic termini and a cDNA coding for the green fluorescence protein (GFP), as a reporter, into a modified pBluescript vector. Co-transfection of the NDV minigenome with three supporting plasmids expressing the N, P, and L proteins into MVA-T7 infected HEp-2 cells and followed by infection with NDV VG/GA resulted in the minigenome replication, transcription, and packaging as evidenced by the reporter gene GFP expression."
According to the news editors, the research concluded: "These results suggest that this LIC approach is a powerful tool for all sequence-independent DNA cloning and multi-DNA fragment assembly, which has a potential application for rapid development of gene therapy and recombinant vaccines."
For more information on this research see: Application of the ligation-independent cloning (LIC) method for rapid construction of a minigenome rescue system for Newcastle disease virus VG/GA strain. Plasmid, 2013;70(3):314-320. Plasmid can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Plasmid - www.elsevier.com/wps/product/cws_home/622933)
Our news journalists report that additional information may be obtained by contacting W. Zhao, Southwest University, Coll Anim Sci & Technol, Chongqing 400715, People's Republic of China. Additional authors for this research include H.X. Hu, L. Zsak, Q.Z. Yu and Z.Q. Yang (see also Animal Diseases).
Keywords for this news article include: Asia, Biotechnology, Cloning, Virology, Chongqing, Viral DNA, RNA Viruses, Gene Therapy, Bird Diseases, Bioengineering, Animal Diseases, Newcastle Disease, Avulavirus Infections, People's Republic of China, Paramyxoviridae Infections
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