By a News Reporter-Staff News Editor at Life Science Weekly -- Fresh data on DNA Research are presented in a new report. According to news reporting originating in Changsha, People's Republic of China, by NewsRx journalists, research stated, "Aptamer-based fluorescence anisotropy (FA) assays have attracted great interest in recent years. However, a key factor that determines FA value is molar mass, thus limiting the utility of this assay for the detection of small molecules."
The news reporters obtained a quote from the research from Hunan University, "To solve this problem, streptavidin, as a molar mass amplifier, was used in a hybridization chain reaction (HCR) to construct a target-triggered cyclic assembly of DNA protein hybrid nanowires for highly sensitive detection of small molecules by fluorescence anisotropy. In this assay, one blocking DNA strand is released by target aptamer recognition. The DNA then serves as an initiator to trigger enzyme-free autonomous cross-opening of hairpin probes via HCR to form a DNA nanowire for further assembly of streptavidin. Using adenosine triphosphate (ATP) as the small molecule target, this novel dual-amplified, aptamer-based FA assay affords high sensitivity with a detection limit of 100 nM. This limit of detection (LOD) is much lower than that of the disassembly approach without HCR amplification or the assembly strategy without streptavidin. In contrast to the previous turn-off disassembly approaches based on nonspecific interactions between the aptamer probe and amplification moieties, the proposed aptamer-based FA assay method exhibits a turn-on response to ATP, which can increase sensing reliability and reduce the risk of false hits."
According to the news reporters, the research concluded: "Moreover, because of its resistance to environmental interferences, this FA assay has been successfully applied for direct detection of 0.5 mu M ATP in complex biological samples, including cell media, human urine, and human serum, demonstrating its practicality in real complex biological systems."
For more information on this research see: Target-Triggered Cyclic Assembly of DNA-Protein Hybrid Nanowires for Dual-Amplified Fluorescence Anisotropy Assay of Small Molecules. Analytical Chemistry, 2013;85(23):11518-11523. Analytical Chemistry can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Analytical Chemistry - www.pubs.acs.org/journal/ancham)
Our news correspondents report that additional information may be obtained by contacting B. Yang, Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Collaborat Innovat Center Chem & Mol MedColl Biol, Coll Chem & Chem EngnMol Sci & Biomed Lab, Changsha 410082, Hunan, People's Republic of China. Additional authors for this research include X.B. Zhang, L.P. Kang, G.L. Shen, R.Q. Yu and W.H. Tan (see also DNA Research).
Keywords for this news article include: Asia, Changsha, Nanowire, DNA Research, Streptavidin, Nanotechnology, Bacterial Proteins, Emerging Technologies, People's Republic of China
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