By a News Reporter-Staff News Editor at Angiogenesis Weekly -- Current study results on Proteins have been published. According to news originating from Wuhan, People's Republic of China, by NewsRx correspondents, research stated, "Schwann cell (SC), which plays a key role in peripheral nerve regeneration, is one of the most classic supportive cells in neural tissue engineering. However, the biological activity of SCs seeded in nerve scaffolds decays subsequently due to local hypoxia induced by ischemia."
Our news journalists obtained a quote from the research from General Hospital, "Thus, we aimed to investigate whether a synthetic oxygen carrier-enriched fibrin gel would provide a sustained oxygen release to cultured SCs in vitro for overcoming a temporary (48 h) oxygen deprivation. In this study, perfluorotributylamine (PFTBA)-based oxygen carrying fibrin gel was prepared to provide oxygen for SCs under normoxic or hypoxic conditions. The dissolved oxygen within the culture media was measured by a blood-gas analyzer to quantify the time course of oxygen release from the PFTBA-enriched fibrin gel. SCs were cultured in the presence or absence of PFTBA-enriched fibrin gel under normoxic or hypoxic conditions. The tolerance of SCs to hypoxia was examined by a cell apoptosis assay. The growth of cells was characterized using S-100 staining and a CCK-8 assay. The migration of cells was examined using a Transwell chamber. The mRNA of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), glial cell derived neurotrophic factor (GDNF), neural cell adhesion molecule (N-CAM) and vascular endothelial growth factor (VEGF) in SCs were assayed by RT-PCR. In addition, SCs cultured in 3D PFTBA-enriched hydrogel were characterized by Live/Dead staining and the mRNA levels of BDNF, NGF, GDNF, NCAM and VEGF were assayed by RT-PCR. The results showed that the PFTBA-enriched fibrin hydrogel was able to promote cell adhesion, migration, and proliferation under hypoxic conditions. Interestingly, PFTBA applied through the fibrin hydrogel dramatically enhanced the mRNA of BDNF, NGF, GDNF, N-CAM and VEGF under hypoxic condition."
According to the news editors, the research concluded: "These findings highlight the possibility of enhancing nerve regeneration in cellular nerve grafts through PFTBA increased neurotropic secretion in SCs."
For more information on this research see: The effect of synthetic oxygen carrier-enriched fibrin hydrogel on Schwann cells under hypoxia condition in vitro. Biomaterials, 2013;34(38):10016-10027. Biomaterials can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Biomaterials - www.elsevier.com/wps/product/cws_home/30392)
The news correspondents report that additional information may be obtained from T. Ma, Wuhan Gen Hosp, Guangzhou Military Area Command, Dept. of Orthopaed, Wuhan 430070, People's Republic of China. Additional authors for this research include Y.Q. Wang, F.Y. Qi, S. Zhu, L.L. Huang, Z.Y. Liu, J.H. Huang and Z.J. Luo (see also Proteins).
Keywords for this news article include: Asia, VEGF, Tissue Engineering, Biomedical Engineering, Biomedicine, Wuhan, Fibrin, Alcohols, Hydrogel, Neuroglia, Chalcogens, Schwann Cells, Bioengineering, Blood Proteins, Protein Kinases, Membrane Proteins, Organic Chemicals, Angiogenic Proteins, Nerve Growth Factor, Phosphotransferases, Polyethylene Glycols
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