By a News Reporter-Staff News Editor at Cancer Weekly -- Investigators publish new report on Proteins. According to news reporting from Hong Kong, People's Republic of China, by NewsRx journalists, research stated, "Based on a recently reported ultra-sensitive bio-barcode (BBC) assay, we have developed an aptamer-based bio-barcode (ABC) alternative to detect a cell death marker cytochrome-c (Cyto-c) and its subsequent application to screen anti-cancer drugs. Aptamer is a short single-stranded DNA selected from a synthetic DNA library by virtue of its high binding affinity and specificity to its target based on its unique 3D structure from the nucleotide sequence after folding."
The news correspondents obtained a quote from the research from the Chinese University of Hong Kong, "In the BBC assay, an antigen (Ag) in analytes is captured by a micro-magnetic particle (MMP) coated with capturing antibodies (Abs). Gold nanoparticles (NPs) with another recognition Ab against the same target and hundreds of identical DNA molecules of known sequence are subsequently added to allow the formation of sandwich structures ([MMP-Ab1]-Ag-[Ab2-NP-DNA]). After isolating the sandwiches by a magnetic field, the DNAs hybridized to their complementary DNAs covalently bound on the NPs are released from the sandwiches after heating. Acting as an Ag identification tag, these bio-barcode DNAs with known DNA sequence are then amplified by polymerase chain reaction (PCR) and detected by fluorescence. In our ABC assay, we employed a Cyto-c-specific aptamer to substitute both the recognition Ab and barcode DNAs on the NPs in the BBC assay; and a novel isothermal recombinase polymerase amplification for the time-consuming PCR."
According to the news reporters, the research concluded: "The detection limit of our ABC assay for the Cyto-c was found to be 10 ng/mL and this new assay can be completed within 3 h. Several potential anti-cancer drugs have been tested in vitro for their efficacy to kill liver cancer with or without multi-drug resistance."
For more information on this research see: An aptamer-based bio-barcode assay with isothermal recombinase polymerase amplification for cytochrome-c detection and anti-cancer drug screening. Talanta, 2013;115():159-165. Talanta can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; Talanta - www.elsevier.com/wps/product/cws_home/525438)
Our news journalists report that additional information may be obtained by contacting J.F.C. Loo, Chinese University of Hong Kong, Dept. of Elect Engn, Center Adv Res Photon, Shatin, Hong Kong, People's Republic of China. Additional authors for this research include P.M. Lau, H.P. Ho and S.K. Kong (see also Proteins).
Keywords for this news article include: Asia, Cancer, Oncology, Hong Kong, Polymerase, Cytochromes, DNA Research, Hemeproteins, Recombinases, Enzymes and Coenzymes, People's Republic of China
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