Because ESCs have the potential to differentiate into cells of any type, they have been the focus of intense interest as possible new treatments for incurable diseases. However, maintenance of mouse or human ESCs in culture requires highly specialized bioreagents, including the human cytokine LIF, that are expensive to produce and that represent an economic barrier to commercial-scale development of ESC-based therapies. InVitria’s parent company, Ventria Bioscience, has developed a highly efficient method for producing rhLIF in a plant-based expression system, ExpressTec. The end product is a highly pure rhLIF that is free of animal components and suitable for use with human and mouse ESCs, induced pluripotent stem cells, and human neural stem cells.
In the present study, the authors evaluated the potency and activity of rhLIF produced using the ExpressTec system in mouse ESC culture. They confirmed that this form of rhLIF was capable of supporting the growth and proliferation of cultured mouse ESCs in an undifferentiated state and that its potency and specific activity were indistinguishable from other commercially available recombinant LIF. Further, rhLIF from InVitria contained lower levels of endotoxin contamination than other sources.
“It is estimated that up to 90 percent of the cost of maintaining stem cell cultures can be attributed to the cost of LIF, and that makes cost-effective commercial scale-up very challenging,” said
The publication resulted from a collaboration between researchers in the
InVitria (www.InVitria.com) develops, manufactures and markets a portfolio of animal-free cell culture supplements that improve performance, cost-effectiveness, and consistency of cell-based biomanufacturing systems. InVitria is a division of Ventria Bioscience.
About Ventria Bioscience
Ventria Bioscience develops, manufactures and markets biotherapeutics and bioreagents. Ventria Bioscience’s patented ExpressTec biomanufacturing technology enables the development of new and cost-effective biologic products. For more information, visit www.Ventria.com.
Source: Ventria Bioscience