By a News Reporter-Staff News Editor at Science Letter -- Current study results on Science have been published. According to news reporting out of Daejeon, South Korea, by NewsRx editors, research stated, "Poor specificity has been a lingering problem in many microRNA profiling methods, particularly surface hybridization-based methods such as microarrays. Here, we carefully investigated surface hybridization and dissociation processes of a number of sequentially similar microRNAs against nucleic acid capture probes."
Our news journalists obtained a quote from the research from the Korea Research Institute of Bioscience and Biotechnology, "Single-base mismatched microRNAs were similarly hybridized to a complementary DNA capture probe and thereby poorly discriminated during conventional stringent hybridization. Interestingly, however, mismatched microRNAs showed significantly faster dissociation from the probe than the perfectly matched microRNA. Systematic analysis of various washing conditions clearly demonstrated that extremely high specificity can be obtained by releasing non-specific microRNAs from assay surfaces during a stringent and controlled dissociation step. For instance, compared with stringent hybridization, surface dissociation control provided up to 6-fold better specificity for Let-7a detection than for other Let-7 family microRNAs. In addition, a synthetically introduced single-base mismatch on miR206 was almost completely discriminated by optimized surface dissociation of captured microRNAs, while this mismatch was barely distinguished from target miR206 during stringent hybridization. Furthermore, a single dissociation condition was successfully used to simultaneously measure four different microRNAs with extremely high specificity using melting temperature-equalized capture probes."
According to the news editors, the research concluded: "The present study on selective dissociation of surface bound microRNAs can be easily applied to various hybridization based detection methods for improved specificity."
For more information on this research see: Highly improved specificity for hybridization-based microRNA detection by controlled surface dissociation. Analyst, 2014;139(1):259-65. (Royal Society of Chemistry - www.rsc.org/; Analyst - pubs.rsc.org/en/journals/journalissues/an)
Our news journalists report that additional information may be obtained by contacting H.R. Yoon, BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Daejeon 305-600, South Korea. Additional authors for this research include J.M. Lee, J. Jung, C.S. Lee, B.H. Chung and Y. Jung (see also Science).
Keywords for this news article include: Asia, Daejeon, Science, South Korea.
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