By a News Reporter-Staff News Editor at Veterinary Week -- Current study results on Biotechnology have been published. According to news reporting from Selangor, Malaysia, by VerticalNews journalists, research stated, "We had examined the immunogenicity of a series of plasmid DNAs which include neuraminidase (NA) and nucleoprotein (NP) genes from avian influenza virus (AIV). The interleukin-15 (IL-15) and interleukin-18 (IL-18) as genetic adjuvants were used for immunization in combination with the N1 and NP AIV genes."
The news correspondents obtained a quote from the research from the University of Tunku Abdul Rahman, "In the first trial, 8 groups of chickens were established with 10 specific-pathogen-free (SPF) chickens per group while, in the second trial 7 SPF chickens per group were used. The overall N1 enzyme-linked immunosorbent assay (ELISA) titer in chickens immunized with the pDis/N1 + pDis/IL-15 was higher compared to the chickens immunized with the pDis/N1 and this suggesting that chicken IL-15 could play a role in enhancing the humoral immune response. Besides that, the chickens that were immunized at 14-day-old (Trial 2) showed a higher N1 antibody titer compared to the chickens that were immunized at 1-day-old (Trial 1). Despite the delayed in NP antibody responses, the chickens co-administrated with IL-15 were able to induce earlier and higher antibody response compared to the pDis/NP and pDis/NP + pDis/IL-18 inoculated groups. The pDis/N1 + pDis/IL-15 inoculated chickens also induced higher CD8+ T cells increase than the pDis/N1 group in both trials (P < 0.05). The flow cytometry results from both trials demonstrated that the pDis/N1 + pDis/IL-18 groups were able to induce CD4+ T cells higher than the pDis/N1 group (P < 0.05). Meanwhile, pDis/N1 + pDis/IL-18 group was able to induce CD8+ T cells higher than the pDis/N1 group (P < 0.05) in Trial 2 only. In the present study, pDis/NP was not significant (P > 0.05) in inducing CD4+ and CD8+ T cells when co-administered with the pDis/IL-18 in both trials in comparison to the pDis/NP."
According to the news reporters, the research concluded: "Our data suggest that the pDis/N1 + pDis/IL-15 combination has the potential to be used as a DNA vaccine against AIV in chickens."
For more information on this research see: Antibody and T cell responses induced in chickens immunized with avian influenza virus N1 and NP DNA vaccine with chicken IL-15 and IL-18. Research in Veterinary Science, 2013;95(3):1224-1234. Research in Veterinary Science can be contacted at: Elsevier Sci Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, Oxon, England. (Elsevier - www.elsevier.com; Research in Veterinary Science - www.elsevier.com/wps/product/cws_home/623070)
Our news journalists report that additional information may be obtained by contacting K.L. Lim, Univ Tunku Abdul Rahman, Fac Med & Hlth Sci, Kajang 43000, Selangor, Malaysia. Additional authors for this research include S.D. Jazayeri, S.K. Yeap, N.B.M. Alitheen, M.H. Bejo, A. Ideris and A.R. Omar.
Keywords for this news article include: Asia, Antibodies, Biotechnology, Viruses, Selangor, Malaysia, Bird Flu, Genetics, Virology, Viral DNA, Immunology, DNA Research, DNA Vaccines, Bird Diseases, Blood Proteins, Avian Influenza, Immunoglobulins, Poultry Diseases, Influenza in Birds, Synthetic Vaccines, RNA Virus Infections, Orthomyxoviridae Infections
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