By a News Reporter-Staff News Editor at Science Letter -- Current study results on Proteobacteria have been published. According to news reporting originating from Wuxi, People's Republic of China, by NewsRx correspondents, research stated, "A sensitive, specific method for the collection and detection of pathogenic bacteria was demonstrated using quantum dots (QDs) as a fluorescence marker coupled with aptamers as the molecular recognition element by flow cytometry. The aptamer sequences were selected using a bacterium-based SELEX strategy in our laboratory for Vibrio parahaemolyticus and Salmonella typhimurium that, when applied in this method, allows for the specific recognition of the bacteria from complex mixtures including shrimp samples."
Our news editors obtained a quote from the research from Jiangnan University, "Aptamer-modified QDs (QD-apt) were employed to selectively capture and simultaneously detect the target bacteria with high sensitivity using the fluorescence of the labeled QDs. The signal intensity is amplified due to the high photostability of QDs nanoparticles, resulting in improved sensitivity over methods using individual dye-labeled probes. This proposed method is promising for the sensitive detection of other pathogenic bacteria in food stuff if suitable aptamers are chosen."
According to the news editors, the research concluded: "The method may also provide another potential platform for the application of aptamer-conjugated QDs in flow cytometry."
For more information on this research see: A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels. Analytica Chimica Acta, 2013;804():151-8. (Elsevier - www.elsevier.com; Analytica Chimica Acta - www.elsevier.com/wps/product/cws_home/502681)
The news editors report that additional information may be obtained by contacting N. Duan, State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Taiwan. Additional authors for this research include S. Wu, Y. Yu, X. Ma, Y. Xia, X. Chen, Y. Huang and Z. Wang (see also Proteobacteria).
Keywords for this news article include: Wuxi, Asia, Cytometry, Quantum Dots, Vibrionaceae, Salmonellosis, Nanotechnology, Quantum Physics, Enterobacteriaceae, Gammaproteobacteria, Salmonella enterica, Emerging Technologies, Gram Negative Bacteria, Salmonella typhimurium, Vibrio parahaemolyticus, People's Republic of China.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC