By a News Reporter-Staff News Editor at Health & Medicine Week -- Research findings on Endocytosis are discussed in a new report. According to news reporting from Cambridge, Massachusetts, by NewsRx journalists, research stated, "Cationic nanoparticles (NPs) and cell-penetrating peptides (CPPs) can enter cells in an energy-independent fashion, escaping the traditional endocytosis route, which is known as direct translocation. This unconventional entry, usually complementary to endocytosis, features rapid uptake and thus makes both cationic NPs and CPPs fascinating intracellular delivery agents."
The news correspondents obtained a quote from the research from the Massachusetts Institute of Technology, "However, the mechanisms of the direct translocation of both cationic NPs and CPPs across cell membranes into the cytosol are not understood. Moreover, the relationship between direct translocation and endocytosis is also unclear. Here, using coarse-grained molecular dynamics simulations we show that a model cell membrane generates a nanoscale hole to assist the spontaneous translocation of cationic gold nanoparticles (AuNPs) as well as HIV-1 Tat peptides to the cytoplasm side under a transmembrane ™ potential. After translocation, the AuNPs/Tat peptides move freely in the 'cytoplasm' region and the membrane reseals itself within a microsecond, while the TM potential is strongly diminished. Furthermore, we show that the shape of the cationic object is crucial in determining if it can translocate or not across. The results provide insights into the uptake kinetics of cationic NPs/CPPs, which features the relationship between direction translocation and endocytosis."
According to the news reporters, the research concluded: "The mechanism put forward here establishes fundamental principles of the intracellular delivery of cationic nanocarriers."
For more information on this research see: Cell Membranes Open "Doors" for Cationic Nanoparticles/Biomolecules: Insights into Uptake Kinetics. ACS Nano, 2013;7(12):10799-10808. ACS Nano can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Nano - www.pubs.acs.org/journal/ancac3)
Our news journalists report that additional information may be obtained by contacting J.Q. Lin, MIT, Dept. of Mat Sci & Engn, Cambridge, MA 02139, United States (see also Endocytosis).
Keywords for this news article include: Peptides, Proteins, Cambridge, Amino Acids, Endocytosis, Nanoparticle, Massachusetts, United States, Cell Membrane, Nanotechnology, Cellular Structures, Emerging Technologies, North and Central America
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