By a News Reporter-Staff News Editor at Health & Medicine Week -- Data detailed on Proteobacteria have been presented. According to news reporting originating in Changsha, People's Republic of China, by NewsRx journalists, research stated, "A simple, selective, sensitive and label-free fluorescent method for detecting trpS-harboring Salmonella typhimurium was developed in this study. This assay used the non-covalent interaction of single-stranded DNA (ssDNA) probes with SWNTs, since SWNTs can quench fluorescence."
The news reporters obtained a quote from the research from Hunan Normal University, "Fluorescence recovery (78% with 1.8 nM target DNA) was detected in the presence of target DNA as ssDNA probes detached from SWNTs hybridized with target DNA, and the resulting double-stranded DNA (dsDNA) intercalated with SYBR Green I (SG) dyes. The increasing fluorescence intensity reached 4.54-fold. In contrast, mismatched oligonucleotides (1-or 3-nt difference to the target DNA) did not contribute to significant fluorescent recovery, which demonstrated the specificity of the assay. The increasing fluorescence intensity increased 3.15-fold when purified PCR products containing complementary sequences of trpS gene were detected."
According to the news reporters, the research concluded: "These results confirmed the ability to use this assay for detecting real samples."
For more information on this research see: Novel strategy combining SYBR Green I with carbon nanotubes for highly sensitive detection of Salmonella typhimurium DNA. Enzyme and Microbial Technology, 2014;54():15-9. (Elsevier - www.elsevier.com; Enzyme and Microbial Technology - www.elsevier.com/wps/product/cws_home/525004)
Our news correspondents report that additional information may be obtained by contacting P. Mao, The Co-construction Laboratory of Microbial Molecular Biology of Province and Ministry of Science and Technology, College of Life Science, Hunan Normal University, Changsha, Hunan, People's Taiwan. Additional authors for this research include Y. Ning, W. Li, Z. Peng, Y. Chen and L. Deng (see also Proteobacteria).
Keywords for this news article include: Asia, Changsha, Fullerenes, DNA Research, Salmonellosis, Nanotechnology, Carbon Nanotubes, Enterobacteriaceae, Gammaproteobacteria, Salmonella enterica, Emerging Technologies, Gram Negative Bacteria, Salmonella typhimurium, People's Republic of China, Gram Negative Facultatively Anaerobic Rods.
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