By a News Reporter-Staff News Editor at AIDS Weekly -- Fresh data on Immune System Diseases and Conditions are presented in a new report. According to news reporting out of Ljubljana, Slovenia, by NewsRx editors, research stated, "To effectively fight against the human immunodeficiency virus infection/ acquired immunodeficiency syndrome (HIV/AIDS) epidemic, ongoing development of novel HIV protease inhibitors is required. Inexpensive high-throughput screening assays are needed to quickly scan large sets of chemicals for potential inhibitors."
Our news journalists obtained a quote from the research from the National Institute of Chemistry, "We have developed a Forster resonance energy transfer (FRET)-based, HIV protease-sensitive sensor using a combination of a fluorescent protein pair, namely mCerulean and mCitrine. Through extensive in vitro characterization, we show that the FRET-HIV sensor can be used in HIV protease screening assays. Furthermore, we have used the FRET-HIV sensor for intracellular quantitative detection of HIV protease activity in living cells, which more closely resembles an actual viral infection than an in vitro assay. We have developed a high-throughput method that employs a ratiometric flow cytometry for analyzing large populations of cells that express the FRET-HIV sensor. The method enables FRET measurement of single cells with high sensitivity and speed and should be used when subpopulation-specific intracellular activity of HIV protease needs to be estimated."
According to the news editors, the research concluded: "In addition, we have used a confocal microscopy sensitized emission FRET technique to evaluate the usefulness of the FRET-HIV sensor for spatiotemporal detection of intracellular HIV protease activity."
For more information on this research see: Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry. Sensors, 2013;13(12):16330-46. (Elsevier - www.elsevier.com; Sensors - www.elsevier.com/wps/product/cws_home/504103)
Our news journalists report that additional information may be obtained by contacting R. Gaber, Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana 1000, Slovenia. Additional authors for this research include A. Majerle, R. Jerala and M. Ben?ina (see also Immune System Diseases and Conditions).
Keywords for this news article include: Europe, Slovenia, HIV/AIDS, Ljubljana, Cytometry, RNA Viruses, HIV Protease, Retroviridae, HIV Infections, Peptide Hydrolases, Vertebrate Viruses, Primate Lentiviruses, Enzymes and Coenzymes, Aspartic Acid Endopeptidases, Human Immunodeficiency Virus, Viral Sexually Transmitted Diseases.
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