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Researchers from Zhengzhou University Report on Findings in Cancer Gene Therapy

February 3, 2014



By a News Reporter-Staff News Editor at Cancer Gene Therapy Week -- A new study on Biotechnology is now available. According to news reporting originating in Zhengzhou, People's Republic of China, by NewsRx journalists, research stated, "To evaluate the impact of Bmi-1 on cell senescence and metastasis of human gastric cancer cell line BGC823. Two pairs of complementary small hairpin RNA (shRNA) oligonucleotides targeting the Bmi-1 gene were designed, synthesized, annealed and cloned into the pRNAT-U6.2 vector."

The news reporters obtained a quote from the research from Zhengzhou University, "After DNA sequencing to verify the correct insertion of the shRNA sequences, the recombinant plasmids were transfected into BGC823 cells. The expression of Bmi-1 mRNA and protein was examined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The effects of Bmi-1 knockdown on cell senescence and metastasis were determined by the beta-Gal activity assay and Boyden chamber assay, respectively. The double-stranded oligonucleotide fragments of Bmi-1 short interfering RNA (siRNA) cloned into pRNAT-U6.2 vector conformed to the inserted sequence. RT-PCR and Western blotting indicated that the expression levels of Bmi-1 gene mRNA and protein were markedly decreased in transfected BGC823 cells with pRNAT-U6.2-si1104 and pRNAT-U6.2-si1356, especially in transfected BGC823 cells with pRNAT-U6.2-si1104, compared with two control groups (empty vector and blank group). In particular, Bmi-1 protein expression was almost completely abolished in cells transfected with the recombinant vector harboring shRNA targeting the sequence GGAGGAGGTGAATGATAAA (nt1104-1122). Compared with untransfected cells and cells transfected with the empty vector, the mean percentage of senescent cells increased and the number of cells passing through the Matrigel decreased in cells transfected with the recombinant vectors."

According to the news reporters, the research concluded: "Silencing Bmi-1 by RNA interference can increase the senescent cell rate and effectively reduce the metastasis of gastric cancer cells."

For more information on this research see: Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells. World Journal of Gastroenterology, 2013;19(46):8764-8769. World Journal of Gastroenterology can be contacted at: Baishideng Publ Grp Co Ltd, Room 1701, 17-F, Henan Building, No. 90, Jaffe Rd, Wanchai, Hong Kong 100025, Peoples R China. (Baishideng Publishing Group - www.wjgnet.com/; World Journal of Gastroenterology - www.wjgnet.com/1007-9327/current.htm)

Our news correspondents report that additional information may be obtained by contacting F.L. Gao, Zhengzhou Univ, Inst Basic Med Sci, Zhengzhou 450001, Henan Province, People's Republic of China. Additional authors for this research include W.S. Li, C.L. Liu and G.Q. Zhao (see also Biotechnology).

Keywords for this news article include: Asia, Biotechnology, Oncology, Zhengzhou, Gastric Cancer, Gastroenterology, Cancer Gene Therapy, People's Republic of China

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Source: Cancer Gene Therapy Week


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