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Research Conducted at Southwest University of Science and Technology Has Updated Our Knowledge about Nucleoproteins

February 6, 2014

By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Fresh data on Proteins are presented in a new report. According to news reporting out of Mianyang, People's Republic of China, by NewsRx editors, research stated, "The liquid crystalline state is a universal phenomenon involving the formation of an ordered structure via a self-assembly process that has attracted attention from numerous scientists. In this study, the dinoflagellate histone-like protein HCcp3 is shown to induce super-coiled pUC18 plasmid DNA to enter a liquid crystalline state in vitro, and the role of HCcp3 in gene condensation in vivo is also presented."

Our news journalists obtained a quote from the research from the Southwest University of Science and Technology, "The plasmid DNA (pDNA)-HCcp3 complex formed birefringent spherical particles with a semi-crystalline selected area electronic diffraction (SAED) pattern. Circular dichroism (CD) titrations of pDNA and HCcp3 were performed. Without HCcp3, pUC18 showed the characteristic B conformation. As the HCcp3 concentration increased, the 273 nm band sharply shifted to 282 nm. When the HCcp3 concentration became high, the base pair (bp)/dimer ratio fell below 42/1, and the CD spectra of the pDNA-HCcp3 complexes became similar to that of dehydrated A-form DNA. Microscopy results showed that HCcp3 compacted the super-coiled gene into a condensed state and that inclusion bodies were formed. Our results indicated that HCcp3 has significant roles in gene condensation both in vitro and in histone-less eukaryotes in vivo."

According to the news editors, the research concluded: "The present study indicates that HCcp3 has great potential for applications in non-viral gene delivery systems, where HCcp3 may compact genetic material to form liquid crystals."

For more information on this research see: A histone-like protein induces plasmid DNA to form liquid crystals in vitro and gene compaction in vivo. International Journal of Molecular Sciences, 2013;14(12):23842-57 (see also Proteins).

Our news journalists report that additional information may be obtained by contacting S. Sun, Key Laboratory of Solid Waste Treatment and Resource Recycle & Fundamental Science on Nuclear Waste and Environmental Security Laboratory, Southwest University of Science and Technology, Mianyang 621010, Sichuan, People's Taiwan. Additional authors for this research include M. Liu, F. Dong, S. Fan and Y. Yao.

Keywords for this news article include: Asia, Biotechnology, Mianyang, Histones, DNA Research, Gene Therapy, Bioengineering, Nucleoproteins, People's Republic of China.

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Source: Gene Therapy Weekly

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