By a News Reporter-Staff News Editor at Health & Medicine Week -- Fresh data on Proteins are presented in a new report. According to news reporting out of Fuzhou, People's Republic of China, by NewsRx editors, research stated, "Prostate-specific antigen (PSA), one of the indications of possible prostate malignancy, is used as a biomarker for the diagnosis and prognosis of prostate cancer. Herein, we develop a new homogeneous potentiometric immunoassay for sensitive detection of low-concentration PSA without the need of sample separation and washing step."
Our news journalists obtained a quote from the research from Fuzhou University, "Two nanostructures including positively charged polyethyleneimine-poly(styrene-co-acrylic acid) (PEI-PSAA) nanospheres and negatively charged gold nanoparticles conjugated with anti-PSA antibody (Ab-AuNP) were first synthesized by using mulsifier-free emulsion copolymerization and wet chemistry method, respectively. Thereafter, the as-prepared PEI-PSAA was used as a pseudo hapten for the construction of immunosensing probe based on an electrostatic interaction between PEI-PSAA and Ab-AuNP. Upon target introduction, the added PSA competed with PEI-PASS for Ab-AuNP based on a specific antigen-antibody interaction, and displaced Ab-AuNP from PEI-PASS. The dissociated PEI-PASS was captured through the negatively charged Nafion-modified electrode, thereby resulting in the change of membrane potential. The fabrication process was characterized by using high-resolution transmission electron microscope (HRTEM), scanning electron microscope with energy-dispersive X-ray spectroscopy (SEM-EDX), surface plasmon resonance (SPR) and dynamic laser scattering (DLS) technique. Under optimal conditions, the output signal was indirectly proportional to the concentration of target PSA in the sample and exhibited a dynamic range from 0.1 to 50 ng/mL with a detection limit (LOD) of 0.04 ng/mL. Intra-and inter-assay coefficients of variation (CVs) were 6.8 and 7.5%, respectively."
According to the news editors, the research concluded: "In addition, the methodology was evaluated for analysis of 12 clinical serum samples and showed good accordance between the results obtained by the developed immunosensing protocol and a commercialized enzyme-linked immunosorbent assay (ELISA) method."
For more information on this research see: Competitive-type displacement reaction for direct potentiometric detection of low-abundance protein. Biosensors & Bioelectronics, 2014;53():465-71. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting B. Zhang, Key Laboratory of Analysis and Detection for Food Safety, Ministry of Education & Fujian Province, Dept. of Chemistry, Fuzhou University, Fuzhou 350108, People's Taiwan. Additional authors for this research include B. Liu, G. Chen and D. Tang (see also Proteins).
Keywords for this news article include: Asia, Antibodies, Fuzhou, Immunology, Blood Proteins, Immunoglobulins, Prostate Specific Antigen, People's Republic of China.
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