By a News Reporter-Staff News Editor at Angiogenesis Weekly -- New research on Oncology is the subject of a report. According to news reporting originating in Guangdong, People's Republic of China, by NewsRx journalists, research stated, "Tumor angiogenesis is a complicated process based upon a sequence of interactions between tumor and vessel endothelial cells. Tumor conditioned medium has been widely used to stimulate endothelial cells in vitro angiogenesis."
The news reporters obtained a quote from the research from the University of Jinan, "This work was aimed to investigate the effects of gold nanoparticles (GNPs) on angiogenesis in hepatic carcinoma-conditioned endothelial cells. Human umbilical vein endothelial cells (HUVECs) were cultured with conditioned medium (CM) from the human hepatocarcinoma cell line HepG2 (HepG2-CM), and then treated with different concentrations of GNPs. The effects of GNPs on the viability, migration and active VEGF level of HUVECs were investigated by MTT assay, wound healing assay and transwell chamber assay, and ELISA assay, respectively. The data showed that GNPs significantly inhibited HUVECs proliferation and migration induced by HepG2-CM, and also reduced the levels of active VEGF in the co-culture system. Then, the alterations in morphology and ultrastructure of HUVECs detected by atomic force microscopy (AFM) showed that there appeared obvious pseudopodia, larger membrane particle sizes and much rougher surface in HUVECs after HepG2-CM treatment, which were all reversed after GNPs treatment. Changes in cytoskeleton of HUVECs determined by immunocytochemistry demonstrated that GNPs treatment remarkably inhibited the activation effect of HepG2-CM on HUVECs, which was associated with the disruption of actin filaments induced by GNPs."
According to the news reporters, the research concluded: "This study indicates that GNPs can significantly inhibit HepG2-CM activated endothelial cell proliferation and migration through down-regulation of VEGF activity and disruption of cell morphology, revealing the potential applications of GNPs as antiangiogenic agent for the treatment of hepatic carcinoma."
For more information on this research see: Inhibition effects of gold nanoparticles on proliferation and migration in hepatic carcinoma-conditioned HUVECs. Bioorganic & Medicinal Chemistry Letters, 2014;24(2):679-684. Bioorganic & Medicinal Chemistry Letters can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Bioorganic & Medicinal Chemistry Letters - www.elsevier.com/wps/product/cws_home/972)
Our news correspondents report that additional information may be obtained by contacting Y.L. Pan, Jinan Univ, Dept. of Histol & Embryol, Guangzhou 510632, Guangdong, People's Republic of China. Additional authors for this research include Q. Wu, R.Y. Liu, M.T. Shao, J. Pi, X.X. Zhao and L. Qin (see also Oncology).
Keywords for this news article include: Asia, VEGF, Oncology, Guangdong, Carcinoma, Treatment, Angiogenesis, Nanoparticle, Hepatic Cancer, Nanotechnology, Protein Kinases, Endothelial Cells, Membrane Proteins, Angiogenic Proteins, Phosphotransferases, Emerging Technologies, Growth Factor Receptors, People's Republic of China, Receptor Protein-Tyrosine Kinases
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