News Column

Reports Outline Gene Therapy Study Findings from State University of New York

February 6, 2014



By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Research findings on Biotechnology are discussed in a new report. According to news reporting originating from Amherst, New York, by NewsRx correspondents, research stated, "Although cellular signaling pathways that affect lentivirus infection have been investigated, the role of cell-cell interactions in lentiviral gene delivery remains elusive. In the course of our studies we observed that lentiviral gene transfer was a strong function of the position of epithelial cells within colonies."

Our news editors obtained a quote from the research from the State University of New York, "While peripheral cells were transduced efficiently, cells in the center of colonies were resistant to gene transfer. In addition, gene delivery was enhanced significantly under culture conditions that disrupted adherens junctions (AJ) but decreased upon AJ formation. In agreement, gene knockdown and gain-of-function approaches showed that ?-catenin, a key component of the AJ complex prevented lentivirus gene transfer. Using a doxycycline regulatable system we showed that expression of dominant negative E-cadherin enhanced gene transfer in a dose-dependent manner. In addition, dissolution of AJ by doxycycline increased entry of lentiviral particles into the cell cytoplasm in a dose-dependent manner."

According to the news editors, the research concluded: "Taken together our results demonstrate that AJ formation renders cells non-permissive to lentiviral gene transfer and may facilitate development of simple means to enhance gene delivery or combat virus infection."

For more information on this research see: Adherens junction formation inhibits lentivirus entry and gene transfer. Plos One, 2013;8(11):e79265. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)

The news editors report that additional information may be obtained by contacting R. Padmashali, Bioengineering Laboratory, Dept. of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, United States. Additional authors for this research include H. You, N. Karnik, P. Lei and S.T Andreadis (see also Biotechnology).

Keywords for this news article include: Biotechnology, Amherst, New York, Gene Therapy, United States, Bioengineering, North and Central America.

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Source: Gene Therapy Weekly


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