The news correspondents obtained a quote from the research, "The K-m was 8.3 and 18.2 mM using catechol and 4-methylcatechol as substrates, respectively. The optimum pH was 7.0 (catechol as the substrate) or 6.5 (4-methylcatechol as the substrate). The optimum temperature was 8 degrees C. The enzyme was stable below 40 degrees C. The activation energy (E-a) of heat inactivation was estimated to be 103.30 kJ/mol. The PPO activity was activated by Mn2+, SDS, Tween-20, Triton X-100, citric acid, and malic acid but inhibited by K+, Zn2+, Mg2+, Ca2+, Ba2+, cetyl trimethyl ammonium bromide (CTAB), kojic acid, tropolone, glutathione (GSH), cysteine (Cys), and ascorbic acid (AA)."
According to the news reporters, the research concluded: "Cys and AA were effective to reduce browning of jackfruit bulbs during the storage at 8 degrees C for 15 days."
For more information on this research see: Purification and Characterization of Polyphenol Oxidase from Jackfruit (Artocarpus heterophyllus) Bulbs.
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