By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in Immunology. According to news reporting out of Seattle, Washington, by NewsRx editors, research stated, "Novel diapedisis method uses shear flow following siRNA knockdown in human monocytes, to improve transendothelial migration. Monocyte recruitment to inflammatory sites and their transendothelial migration into tissues are critical to homeostasis and pathogenesis of chronic inflammatory diseases."
Our news journalists obtained a quote from the research from the University of Washington, "However, even short-term suspension culture of primary human monocytes leads to phenotypic changes. In this study, we characterize the functional effects of ex vivo monocyte culture on the steps involved in monocyte transendothelial migration. Our data demonstrate that monocyte diapedesis is impaired by as little as 4 h culture, and the locomotion step is subsequently compromised. After 16 h in culture, monocyte diapedesis is irreversibly reduced by approximate to 90%. However, maintenance of monocytes under conditions mimicking physiological flow (5-7.5 dyn/cm(2)) is sufficient to reduce diapedesis impairment significantly. Thus, through the application of shear during ex vivo culture of monocytes, our study establishes a novel protocol, allowing functional analyses of monocytes not currently possible under static culture conditions."
According to the news editors, the research concluded: "These data further suggest that monocyte-based therapeutic applications may be measurably improved by alteration of ex vivo conditions before their use in patients."
For more information on this research see: Technical Advance: Novel ex vivo culture method for human monocytes uses shear flow to prevent total loss of transendothelial diapedesis function. Journal of Leukocyte Biology, 2014;95(1):191-195. Journal of Leukocyte Biology can be contacted at: Federation Amer Soc Exp Biol, 9650 Rockville Pike, Bethesda, MD 20814-3998, USA (see also Immunology).
Our news journalists report that additional information may be obtained by contacting Y. Tsubota, University of Washington, Sch Med, Dept. of Pathol, Seattle, WA 98104, United States. Additional authors for this research include J.M. Frey and E.W. Raines.
Keywords for this news article include: Seattle, Monocytes, Washington, Immunology, Blood Cells, Therapeutics, United States, Bone Marrow Cells, Mononuclear Leukocytes, Hemic and Immune Systems, North and Central America, Mononuclear Phagocyte System
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