By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators discuss new findings in Immunology. According to news originating from Belgrade, Serbia, by NewsRx correspondents, research stated, "The protocols for the preparation of dendritic cells (DCs) as cancer vaccines based on stimulation of Toll-like receptors (TLRs) are very promising. In this study we covalently attached 7-thia-8-oxoguanosine (7-TOG), a selective endosomal TLR7, to previously oxidized multi-walled carbon nanotubes (o-MWCNTs) (mass ratio 1:5.6, respectively), and tested their ability to activate human monocyte-derived (Mo)DCs."
Our news journalists obtained a quote from the research from the University of Belgrade, "Light, confocal and transmission electron microscopy confirmed efficient phagocytosis of 7-TOG-MWCNTs by MoDCs and their efficient delivery to TLR7(+) endosomes. The biocompatibility studies showed that 7-TOG-MWCNTs, at concentrations lower than 100 mu g/ml, were not cytotoxic for MoDCs. 7-TOG-MV/CNTs (50 mu g/ml) up-regulated CD86 expression, allostimulatory activity, T helper (Th)1- and Th17-polarizing capability of MoDCs. The same concentration of soluble 7-TOG, alone or in combination with control o-MWCNTs, did not have such effects. It can be hypothesized that the efficacy of 7-TOG-MWCNTs in stimulating MoDCs was a consequence of increased intracellular concentration of 7-TOG after internalization of the nano-complexes, because similar Th-polarizing capability could be induced with 10-times higher concentrations of soluble 7-TOG."
According to the news editors, the research concluded: "Our results suggest that functionalized MWCNTs may be a promising system for the delivery of drugs to intracellular targets, in order to improve the immunogenic potential of DCs for therapeutic purposes."
For more information on this research see: Immunomodulatory effects of carbon nanotubes functionalized with a Toll-like receptor 7 agonist on human dendritic cells. Carbon, 2014;67():273-287. Carbon can be contacted at: Pergamon-Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB, England. (Elsevier - www.elsevier.com; Carbon - www.elsevier.com/wps/product/cws_home/258)
The news correspondents report that additional information may be obtained from M. Colic, University of Belgrade, Fac Technol & Met, Nanotechnol & Funct Mat Center, Belgrade 11120, Serbia. Additional authors for this research include T. Dzopalic, S. Tomic, J. Rajkovic, R. Rudolf, G. Vukovic, A. Marinkovic and P. Uskokovic (see also Immunology).
Keywords for this news article include: Antigen-Presenting Cells, Serbia, Europe, Belgrade, Fullerenes, Immunology, Therapeutics, Nanotechnology, Dendritic Cells, Carbon Nanotubes, Membrane Proteins, Toll-Like Receptors, Emerging Technologies, Mononuclear Phagocyte System, Pattern Recognition Receptors
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