By a News Reporter-Staff News Editor at AIDS Weekly -- Researchers detail new data in Immune System Diseases and Conditions. According to news originating from Fukushima, Japan, by NewsRx correspondents, research stated, "The development of gene therapy techniques to introduce transgenes that promote neuronal survival and protection provides effective therapeutic approaches for neurological and neurodegenerative diseases. Intramuscular injection of adenoviral and adeno-associated viral vectors, as well as lentiviral vectors pseudotyped with rabies virus glycoprotein (RV-G), permits gene delivery into motor neurons in animal models for motor neuron diseases."
Our news journalists obtained a quote from the research from the Fukushima Medical University School of Medicine, "Recently, we developed a vector with highly efficient retrograde gene transfer (HiRet) by pseudotyping a human immunodeficiency virus type 1 (HIV-1)-based vector with fusion glycoprotein B type (FuG-B) or a variant of FuG-B (FuG-B2), in which the cytoplasmic domain of RV-G was replaced by the corresponding part of vesicular stomatitis virus glycoprotein (VSV-G). We have also developed another vector showing neuron-specific retrograde gene transfer (NeuRet) with fusion glycoprotein C type, in which the short C-terminal segment of the extracellular domain and transmembrane/cytoplasmic domains of RV-G was substituted with the corresponding regions of VSV-G. These two vectors afford the high efficiency of retrograde gene transfer into different neuronal populations in the brain. Here we investigated the efficiency of the HiRet (with FuG-B2) and NeuRet vectors for retrograde gene transfer into motor neurons in the spinal cord and hindbrain in mice after intramuscular injection and compared it with the efficiency of the RV-G pseudotype of the HIV-1-based vector."
According to the news editors, the research concluded: "The main highlight of our results is that the HiRet vector shows the most efficient retrograde gene transfer into both spinal cord and hindbrain motor neurons, offering its promising use as a gene therapeutic approach for the treatment of motor neuron diseases."
For more information on this research see: Highly efficient retrograde gene transfer into motor neurons by a lentiviral vector pseudotyped with fusion glycoprotein. Plos One, 2013;8(9):e75896. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news correspondents report that additional information may be obtained from M. Hirano, Dept. of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima, Japan. Additional authors for this research include S. Kato, K. Kobayashi, T. Okada, H. Yaginuma and K. Kobayashi (see also Immune System Diseases and Conditions).
Keywords for this news article include: Asia, Biotechnology, Japan, Viruses, Virology, Fukushima, Gene Therapy, Therapeutics, Glycoproteins, Bioengineering, Glycoconjugates, Immune System Diseases and Conditions.
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