By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Myeloid Cells. According to news reporting originating from Ibaraki, Japan, by NewsRx correspondents, research stated, "Although carbon nanotubes (CNTs) are promising nanomaterials, their potential carcinogenicity is a major concern. We previously established a genetic method of analyzing genotoxicity of chemical compounds, where we evaluated their cytotoxic effect on the DT40 lymphoid cell line comparing DNA-repair-deficient isogenic clones with parental wild-type cells."
Our news editors obtained a quote from the research from National Institute for Environmental Studies, "However, application of our DT40 system for the cytotoxic and genotoxic evaluation of nanomaterials seemed to be difficult, because DT40 cells only poorly internalized nanoparticles. To solve this problem, we have constructed a chimeric gene encoding a trans-membrane receptor consisting of the 5' region of the transferrin receptor (TR) gene (to facilitate internalization of nanoparticles) and the 3' region of the macrophage receptor with collagenous structure (MARCO) gene (which is a receptor for environmental particles). We expressed the resulting MARCO-TR chimeric receptor on DNA-repair-proficient wild-type cells and mutants deficient in base excision repair (FEN1 (-/-)) and translesion DNA synthesis (REV3 (-/-)). We demonstrated that the chimera mediates uptake of particles such as fluorescence-tagged polystyrene particles and multi-walled carbon nanotubes (MWCNTs), with very poor uptake of those particles by DT40 cells not expressing the chimera. MWCNTs were cytotoxic and this effect was greater in FEN1 (-/-)and REV3 (-/-) cells than in wild-type cells. Furthermore, MWCNTs induced greater oxidative damage (measured as 8-OH-dG formation) and a larger number of mitotic chromosomal aberrations in repair-deficient cells compared to repair-proficient cells."
According to the news editors, the research concluded: "Taken together, our novel assay system using the chimeric receptor-expressing DT40 cells provides a sensitive method to screen for genotoxicity of CNTs and possibly other nanomaterials."
For more information on this research see: A novel genotoxicity assay of carbon nanotubes using functional macrophage receptor with collagenous structure (MARCO)-expressing chicken B lymphocytes. Archives of Toxicology, 2014;88(1):145-160. Archives of Toxicology can be contacted at: Springer Heidelberg, Tiergartenstrasse 17, D-69121 Heidelberg, Germany. (Springer - www.springer.com; Archives of Toxicology - www.springerlink.com/content/0340-5761/)
The news editors report that additional information may be obtained by contacting M.o.h.i.u.d.d.i.n., .N.a.t.l. Inst Environm Studies, Environm Nanotoxicol Sect, RCER, Tsukuba, Ibaraki 3058506, Japan. Additional authors for this research include I.S. Keka, T.J. Evans, K. Hirota, H. Shimizu, K. Kono, S. Takeda and S. Hirano (see also Myeloid Cells).
Keywords for this news article include: Asia, Antibody-Producing Cells, Japan, Ibaraki, Fullerenes, Immunology, Blood Cells, Macrophages, Nanoparticle, B-Lymphocytes, Myeloid Cells, Nanotechnology, Carbon Nanotubes, Emerging Technologies, Mononuclear Leukocytes, Connective Tissue Cells, Hemic and Immune Systems, Mononuclear Phagocyte System
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