By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Fresh data on Ischemia are presented in a new report. According to news reporting out of Gif sur Yvette, France, by NewsRx editors, research stated, "The purpose of this study is to further document alteration of signal transduction pathways, more particularly of hedgehog (Hh) signaling, causing impaired ischemic muscle repair in old mice. Approach and Results We used 12-week-old (young mice) and 20- to 24-month-old C57BL/6 mice (old mice) to investigate the activity of Hh signaling in the setting of hindlimb ischemia-induced angiogenesis and skeletal muscle repair."
Our news journalists obtained a quote from the research from Institute for Neurobiology, "In this model, delayed ischemic muscle repair observed in old mice was associated with an impaired upregulation of Gli1. Sonic Hh expression was not different in old mice compared with young mice, whereas desert Hh (Dhh) expression was downregulated in the skeletal muscle of old mice both in healthy and ischemic conditions. The rescue of Dhh expression by gene therapy in old mice promoted ischemia-induced angiogenesis and increased nerve density; nevertheless, it failed to promote myogenesis or to increase Gli1 mRNA expression. After further investigation, we found that, in addition to Dhh, smoothened expression was significantly downregulated in old mice. We used smoothened haploinsufficient mice to demonstrate that smoothened knockdown by 50% is sufficient to impair activation of Hh signaling and ischemia-induced muscle repair. The present study demonstrates that Hh signaling is impaired in aged mice because of Dhh and smoothened downregulation."
According to the news editors, the research concluded: "Moreover, it shows that hegdehog-dependent regulation of angiogenesis and myogenesis involves distinct mechanisms."
For more information on this research see: Hedgehog-Dependent Regulation of Angiogenesis and Myogenesis Is Impaired in Aged Mice. Arteriosclerosis Thrombosis and Vascular Biology, 2013;33(12):2858-2866. Arteriosclerosis Thrombosis and Vascular Biology can be contacted at: Lippincott Williams & Wilkins, 530 Walnut St, Philadelphia, PA 19106-3621, USA (see also Ischemia).
Our news journalists report that additional information may be obtained by contacting M.A. Renault, CNRS, Inst Neurobiol Alfred Fessard IFR2118, Lab Neurobiol & Dev, UPR 3294, Gif Sur Yvette, France. Additional authors for this research include F. Robbesyn, C. Chapouly, Q.Y. Yao, S. Vandierdonck, A. Reynaud, I. Belloc, E. Traiffort, M. Ruat, C. Desgranges and A.P. Gadeau.
Keywords for this news article include: Biotechnology, France, Europe, Ischemia, Angiogenesis, Gene Therapy, Gif sur Yvette, Bioengineering
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