By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on DNA Research have been published. According to news reporting from Boston, Massachusetts, by NewsRx journalists, research stated, "Retinitis pigmentosa (RP) is the most genetically heterogeneous disorder known to cause blindness, involving over 50 different genes. Previously, we have described nanoparticles (NPs) 150 nm in size, comprised of a 3.5 kD peptide (POD) complexed to PEG and DNA (PEGPOD DNA)."
The news correspondents obtained a quote from the research from the Tufts University School of Medicine, "These NPs expressing GDNF enabled rescue of photoreceptor degeneration in mice up to 11 days post injection. In the current study we examine use of scaffold/ matrix attachment regions (S/MARs), CpG depletion and titration of DNA content of PEGPOD DNA NPs to extend the duration of transgene expression. S/MARs and CpGs did not significantly influence the duration of transgene expression, but did influence its stability. These parameters enabled us to extend transgene expression from 48 hours to 10 weeks. At 77 days post injection, we observed a 76% rescue of the thickness of the retinal outer nuclear layer (ONL) and at 37 days post injection we observed 53% and 55% rescue of the A and B wave ERG amplitudes respectively and 60% rescue of the ONL."
According to the news reporters, the research concluded: "Our studies suggest that PEGPOD DNA NPs have potential as gene delivery vectors for the retina."
For more information on this research see: Extended duration of transgene expression from pegylated POD nanoparticles enables attenuation of photoreceptor degeneration. Plos One, 2013;8(11):e82295. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
Our news journalists report that additional information may be obtained by contacting C. Binder, Dept. of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts, United States. Additional authors for this research include S.M. Cashman and R. Kumar-Singh (see also DNA Research).
Keywords for this news article include: Biotechnology, Boston, DNA Research, Gene Therapy, Nanoparticle, Massachusetts, United States, Bioengineering, Nanotechnology, Emerging Technologies, North and Central America.
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