By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on Life Science Research is now available. According to news reporting out of Hiroshima, Japan, by NewsRx editors, research stated, "To develop a microbial production platform based on hydrogen and carbon dioxide, a genetic transformation system for the thermophilic acetogen Moorella thermoacetica ATCC39073 was developed. The uracil auxotrophic strain dpyrF was constructed by disrupting pyrF for orotate monophosphate decarboxylase."
Our news journalists obtained a quote from the research from the National Institute of Advanced Industrial Science and Technology (NIAIST), "The transformation plasmids were methylated by restriction methylases of M. thermoacetica to avoid the decomposition of introduced plasmids by restriction-modification system. Reintroduction of native pyrF into the mutant by homologous recombination ensured recovery from uracil auxotrophy. To test heterologous gene expression in dpyrF, the lactate dehydrogenase (LDH) gene (T-ldh) from Thermoanaerobacter pseudethanolicus ATCC33223 was electroporated into dpyrF with a promoter of the glyceraldehyde-3-phosphate dehydrogenase (G3PD) gene of M. thermoacetica ATCC39073."
According to the news editors, the research concluded: "The resulting transformant (C31) successfully transcribed T-ldh and exhibited higher LDH activity than ATCC39073 and dpyrF, yielding 6.8 mM of lactate from fructose, whereas ATCC39073 did not produce lactate."
For more information on this research see: Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica. Journal of Bioscience and Bioengineering, 2013;115(4):347-52. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news journalists report that additional information may be obtained by contacting A. Kita, Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, Hiroshima, Japan. Additional authors for this research include Y. Iwasaki, S. Sakai, S. Okuto, K. Takaoka, T. Suzuki, S. Yano, S. Sawayama, T. Tajima, J. Kato, N. Nishio, K. Murakami and Y. Nakashimada (see also Life Science Research).
Keywords for this news article include: Asia, Japan, Genetics, Hiroshima, Life Science Research.
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