By a News Reporter-Staff News Editor at Health & Medicine Week -- A new study on Proteobacteria is now available. According to news reporting originating in Changsha, People's Republic of China, by NewsRx journalists, research stated, "A simple, selective, sensitive and label-free fluorescent method for detecting trpS-harboring Salmonella typhimurium was developed in this study. This assay used the non-covalent interaction of single-stranded DNA (ssDNA) probes with SWNTs, since SWNTs can quench fluorescence."
The news reporters obtained a quote from the research from Central South University, "Fluorescence recovery (78% with 1.8 nM target DNA) was detected in the presence of target DNA as ssDNA probes detached from SWNTs hybridized with target DNA, and the resulting double-stranded DNA (dsDNA) intercalated with SYBR Green I (SG) dyes. The increasing fluorescence intensity reached 4.54-fold. In contrast, mismatched oligonucleotides (1- or 3-nt difference to the target DNA) did not contribute to significant fluorescent recovery, which demonstrated the specificity of the assay. The increasing fluorescence intensity increased 3.15-fold when purified PCR products containing complementary sequences of trpS gene were detected."
According to the news reporters, the research concluded: "These results confirmed the ability to use this assay for detecting real samples."
For more information on this research see: Novel strategy combining SYBR Green I with carbon nanotubes for highly sensitive detection of Salmonella typhimurium DNA. Enzyme and Microbial Technology, 2014;54():15-19. Enzyme and Microbial Technology can be contacted at: Elsevier Science Inc, 360 Park Ave South, New York, NY 10010-1710, USA. (Elsevier - www.elsevier.com; Enzyme and Microbial Technology - www.elsevier.com/wps/product/cws_home/525004)
Our news correspondents report that additional information may be obtained by contacting P.D. Mao, Central South University, Coll Life Sci, Changsha, Hunan, People's Republic of China. Additional authors for this research include Y. Ning, W.K. Li, Z.H. Peng, Y.Z. Chen and L. Deng (see also Proteobacteria).
Keywords for this news article include: Asia, Changsha, Fullerenes, DNA Research, Salmonellosis, Nanotechnology, Carbon Nanotubes, Enterobacteriaceae, Gammaproteobacteria, Salmonella enterica, Emerging Technologies, Gram-Negative Bacteria, Salmonella typhimurium, People's Republic of China, Gram-Negative Facultatively Anaerobic Rods
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC