By a News Reporter-Staff News Editor at Health & Medicine Week -- Researchers detail new data in Gram-Positive Bacterial Infections. According to news reporting out of Guangzhou, People's Republic of China, by NewsRx editors, research stated, "Clostridium difficile toxin B (Tcd B), as one of the primary contributing factors to the pathogenesis of C. difficile-associated diseases, has raised serious public concerns due to its virulence, spore-forming ability and persistence with major types of infectious diarrhea diseases, and been used as a potential biomarker in clinical diagnoses. Thus, a simple method for the determination of Tcd B was developed based on a sandwich-type electrochemical immunosensor."
Our news journalists obtained a quote from the research from the South China University of Technology, "Greatly enhanced sensitivity was achieved based on fabricating the immunosensor by layer-by-layer coating carbon nanotubes (MWCNTs), Prussian blue (PB), Chitosan (CS), Glutaraldehyde (GA) composite on the working electrode as well as using graphene oxide (GO) as a nanocarrier in a multienzyme amplification strategy. In comparison with conventional methods, the proposed immunoassay exhibited high sensitivity and selectivity for the detection of Tcd B, providing a better linear response range from 0.003 to 320 ng/mL and a lower limit of detection (LOD) of 0.7 pg/mL (S/N=3) under optimal experimental conditions. The immunosensor exhibited convenience, low cost, rapidity, good specificity, acceptable stability and reproducibility."
According to the news editors, the research concluded: "Moreover, satisfactory results were obtained for the determination of Tcd B in real human stool samples, indicating that the developed immunoassay has the potential to find application in clinical detection of Tcd B and other tumor markers as an alternative approach."
For more information on this research see: Simple approach for ultrasensitive electrochemical immunoassay of Clostridium difficile toxin B detection. Biosensors & Bioelectronics, 2014;53():238-44. (Elsevier - www.elsevier.com; Biosensors & Bioelectronics - www.elsevier.com/wps/product/cws_home/405913)
Our news journalists report that additional information may be obtained by contacting Y.S. Fang, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Taiwan. Additional authors for this research include S.Y. Chen, X.J. Huang, L.S. Wang, H.Y. Wang and J.F Wang (see also Gram-Positive Bacterial Infections).
Keywords for this news article include: Asia, Guangzhou, Chemistry, Electrochemical, Clostridium difficile, Clostridium Infections, People's Republic of China, Gram Positive Bacterial Infections, Gram-Positive Bacterial Infections, Gram Positive Endospore Forming Rods, Gram Positive Endospore Forming Bacteria.
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