By a News Reporter-Staff News Editor at Health & Medicine Week -- Data detailed on Epithelial Cells have been presented. According to news originating from Pretoria, South Africa, by NewsRx correspondents, research stated, "Nanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines."
Our news journalists obtained a quote from the research from the Tshwane University of Technology, "Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines."
According to the news editors, the research concluded: "Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects."
For more information on this research see: Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity. Express Polymer Letters, 2014;8(3):197-206. Express Polymer Letters can be contacted at: Budapest Univ Technol & Econ, Dept Polymer Eng, Muegyetem Rkp 3, Budapest, H-1111, Hungary (see also Epithelial Cells).
The news correspondents report that additional information may be obtained from L.A. Nkabinde, Tshwane Univ Technol, Dept. of Pharmaceut Sci, ZA-0001 Pretoria, South Africa. Additional authors for this research include L.N.N. Shoba-Zikhali, B. Semete-Makokotlela, L. Kalombo, H. Swai, A. Grobler and J.H. Hamman.
Keywords for this news article include: Pretoria, South Africa, Caco-2 Cells, Nanoparticle, Nanotechnology, Epithelial Cells, Emerging Technologies
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC