By a News Reporter-Staff News Editor at Health & Medicine Week -- New research on Health and Medicine is the subject of a report. According to news reporting from Poznan, Poland, by NewsRx journalists, research stated, "To identify potential biomarkers of azoospermia to determine a particular stage of spermatogenetic differentiation. GeneChip Human Gene 1.0 ST microarray with validation at mRNA and protein levels. Basic research laboratory."
The news correspondents obtained a quote from the research from the Institute of Human Genetics, "Men with various types of nonobstructive azoospermia (n=18) and with normal spermatogenesis (n=4). Obtaining 31 testicular biopsy samples. Gene expression analysis using the Affymetrix Human Gene 1.0 ST microarrays on 14 selected genes according to the highest fold change, verified with quantitative polymerase chain reaction and on independent set of microarray samples. Western blot and immunohistochemistry were additionally performed. The comparative analysis of gene expression profiles in the infertile and control groups resulted in the selection of 4,946 differentially expressed genes. AKAP4, UBQLN3, CAPN11, GGN, SPACA4, SPATA3, and FAM71F1 were the most significantly down-regulated genes in infertile patients. Global analysis also led to identification of up-regulated genes-WBSCR28, ADCY10, TMEM225, SPATS1, FSCN3, GTSF1L, and GSG1-in men with late maturation arrest. Moreover, the results from quantitative polymerase chain reaction and Western blot largely confirmed the microarray data."
According to the news reporters, the research concluded: "The set of selected genes can be used to create a molecular diagnostic tool to determine the degree of spermatogenic impairment for men with idiopathic nonobstructive azoospermia."
For more information on this research see: Potential biomarkers of nonobstructive azoospermia identified in microarray gene expression analysis. Fertility and Sterility, 2013;100(6):1686-94.e1-7. (Elsevier - www.elsevier.com; Fertility and Sterility - www.elsevier.com/wps/product/cws_home/600420)
Our news journalists report that additional information may be obtained by contacting A. Malcher, Dept. of Reproductive Biology and Stem Cells, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland. Additional authors for this research include N. Rozwadowska, T. Stokowy, T. Kolanowski, P. Jedrzejczak, W. Zietkowiak and M. Kurpisz (see also Health and Medicine).
Keywords for this news article include: Poznan, Poland, Europe, Machine Learning, Health and Medicine, Emerging Technologies, Gene Expression Analysis.
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC