By a News Reporter-Staff News Editor at Drug Week -- Research findings on Enzymes and Coenzymes are discussed in a new report. According to news originating from Coimbatore, India, by NewsRx correspondents, research stated, "In the present study, we prepared a gelatin nanofiber matrix using an electrospinning technique and cross-linked the nanofibers with 10 % glutaraldehyde vapors. The insoluble nanofibers were functionalized with bioactive molecules like biotin (1 %) and galactose (1 %) by adsorption and coelectrospinning."
Our news journalists obtained a quote from the research from the PSG College of Technology, "Surface morphology and fiber dimension were analyzed using atomic force microscopy. The amounts of biotin and galactose bound to the nanofibers before and after adsorption were quantified using high-performance liquid chromatography. Human larynx carcinoma (HEp-2) cell attachment, morphology and cytotoxic characteristics were studied using crystal violet staining and the MTT assay. Cell attachment and viability were highest in biotin- and galactose-embedded nanofibers compared to native nanofibers. Cytotoxicity was less with biotin- and galactose-embedded and adsorbed nanofibers compared to control nanofibers."
According to the news editors, the research concluded: "Hence, we suggest that these biocompatible, nontoxic, biodegradable, functionalized nanofibers could be a potential candidate for application in tissue engineering and scaffold preparation."
For more information on this research see: Effect of Biotin and Galactose Functionalized Gelatin Nanofiber Membrane on HEp-2 Cell Attachment and Cytotoxicity. Journal of Membrane Biology, 2014;247(1):35-43. Journal of Membrane Biology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Journal of Membrane Biology - www.springerlink.com/content/0022-2631/)
The news correspondents report that additional information may be obtained from R. Selvakumar, PSG Coll Technol, Dept. of Biotechnol, Coimbatore 641004, Tamil Nadu, India. Additional authors for this research include S.N.M. Mohaideen, S. Aravindh, C. Sabarinath and M. Ananthasubramanian (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, India, Biotin, Gelatin, Coimbatore, Micronutrient, Scleroproteins, Diet and Nutrition, Enzymes and Coenzymes
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