By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Fresh data on Enzymes and Coenzymes are presented in a new report. According to news reporting from Yangling, People's Republic of China, by NewsRx journalists, research stated, "BackgroundPhiC31 integrase is capable of conferring long-term transgene expression in various transfected tissues in vivo. In the present study, we investigated the activity of phiC31 integrase in mouse mammary glands."
The news correspondents obtained a quote from the research from Northwest A&F University, "MethodsThe normal mouse mammary epithelial cell line HC11 was transfected with FuGENE ® HD Transfection Reagent (Roche Diagnostics, Shanghai, China). Transfection of the mouse mammary gland in vivo was performed by electrotransfer. Transgene expression was detected by western blotting and an enzyme-linked immunosorbent assay. Genomic integration and integration at mpsL1 was confirmed by a nested polymerase chain reaction. ResultsAn optimal electrotransfer protocol for the lactating mouse mammary gland was attained through investigation of different voltages and pulse durations. PhiC31 integrase mediated site-specific transgene integration in HC11 cells and the mouse mammary gland. In addition, the site-specific integration occurred efficiently at the hot spot' mpsL1. Co-delivery of PhiC31 integrase enhanced and prolonged transgene expression in the mouse mammary gland."
According to the news reporters, the research concluded: "ConclusionsThe results obtained in the present study show that the use of phiC31 integrase is a feasible and efficient method for high and stable transgene expression in the mouse mammary gland."
For more information on this research see: PhiC31 integrase-mediated genomic integration and stable gene expression in the mouse mammary gland after gene electrotransfer. Journal of Gene Medicine, 2013;15(10):356-365. Journal of Gene Medicine can be contacted at: Wiley-Blackwell, 111 River St, Hoboken 07030-5774, NJ, USA. (Wiley-Blackwell - www.wiley.com/; Journal of Gene Medicine - onlinelibrary.wiley.com/journal/10.1002/(ISSN)1521-2254)
Our news journalists report that additional information may be obtained by contacting Y. Luo, Northwest A&F Univ, Key Lab Anim Biotechnol, Coll Vet Med, Minist Agr, Yangling 712100, Shaanxi Provinc, People's Republic of China. Additional authors for this research include J. Liu, Y.S. Wang, J.M. Su, Y.Y. Wu, G.D. Hu, M.Q. Gao, F.S. Quan and Y. Zhang (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Yangling, Integrases, Recombinases, Enzymes and Coenzymes, People's Republic of China
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