By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Biotechnology. According to news originating from Beijing, People's Republic of China, by NewsRx correspondents, research stated, "Nonviral vectors are highly desirable for the development of efficient gene delivery systems. In this study, we report the monomolecular condensation of plasmid DNA and efficient cell transfection by imidazolium gemini surfactants ([C-12-4-C(12)im]Br-2), which could be a potential nonviral vector for efficient gene therapy."
Our news journalists obtained a quote from the research from National Center for Nanoscience and Technology, "Homogeneous DNA/[C-12-4-C(12)im]Br-2 nanoparticles are formed with a diameter of approximately 100 nm and investigated by using atomic force microscopy. DNA condensates evolve from supercoiled DNA molecules, to individual toroids, to close-packed particles, and eventually to multimolecular aggregates with the increase of [C-12-4-C(12)im]Br-2 concentrations. Highly efficient gene transfection in vitro is demonstrated in human embryonic kidney 293 (HEK293) and HeLa cells, which could be attributed to the effective DNA condensation into uniform nanoparticles induced by [C-12-4-C(12)im]Br-2. In addition, the low cytotoxicity of [C-12-4-C(12)im]Br-2 at transfection concentration region verified by cell viability assay (3[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, MTT assay) also supports [C-12-4-C(12)im]Br-2 as an effective gene vector."
According to the news editors, the research concluded: "The high gene transfection efficiency by [C-12-4C(12)im]Br-2 as well as its low cytotoxicity could shed light on the rational molecular design of nonviral vectors for gene delivery systems."
For more information on this research see: High Transfection Efficiency of Homogeneous DNA Nanoparticles Induced by Imidazolium Gemini Surfactant as Nonviral Vector. Journal of Physical Chemistry C, 2013;117(50):26573-26581. Journal of Physical Chemistry C can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; Journal of Physical Chemistry C - www.pubs.acs.org/journal/jpccck)
The news correspondents report that additional information may be obtained from T. Zhou, Natl Center Nanosci & Technol, Beijing 100190, People's Republic of China. Additional authors for this research include A. Llizo, P. Li, C.X. Wang, Y.Y. Guo, M.Q. Ao, L.L. Bai, C. Wang, Y.L. Yang and G.Y. Xu (see also Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Beijing, DNA Research, Gene Therapy, Nanoparticle, Bioengineering, Nanotechnology, Emerging Technologies, People's Republic of China
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