By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Current study results on Biotechnology have been published. According to news reporting out of Taejon, South Korea, by NewsRx editors, research stated, "Polyamidoamine (PAMAM) is one of the widely employed non-viral vectors in gene therapy research, and shows excellent biocompatibility and relatively low cytotoxicity. However, it has poor transfection efficiency compared with that of polyethylenimine (PEI, 25 kDa)."
Our news journalists obtained a quote from the research from Korea Basic Science Institute, "To enhance the gene expression efficiency, we introduced the RRRK peptide from mouse fibroblast growth factor 3 (FGF3) to PAMAM, which is a known nuclear localization signal (NLS). We synthesized PAMAM-KRRR and PAMAM-RRRK to verify the difference of the induced functional status from reversal of the N-terminus. PAMAM containing the FGF3 peptide showed a transfection efficiency corresponding to that of PEI in HEK293, and HeLa cells, and showed much higher gene expression capacity than that of PEI in NIH3T3 cells with relatively decreased cytotoxicity."
According to the news editors, the research concluded: "These results imply that introduction of the FGF 3 peptide has the potential to provide a novel PAMAM-based vector by enhancing its gene expression efficiency."
For more information on this research see: Gene delivery of PAMAM dendrimer conjugated with the nuclear localization signal peptide originated from fibroblast growth factor 3. International Journal of Pharmaceutics, 2014;459(1-2):10-18. International Journal of Pharmaceutics can be contacted at: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands. (Elsevier - www.elsevier.com; International Journal of Pharmaceutics - www.elsevier.com/wps/product/cws_home/505513)
Our news journalists report that additional information may be obtained by contacting J. Lee, Korea Basic Sci Inst, Taejon 305333, South Korea. Additional authors for this research include J. Jung, Y.J. Kim, E. Lee and J.S. Choi (see also technology.html">Biotechnology).
Keywords for this news article include: Asia, Biotechnology, Taejon, Peptides, Proteins, Proteomics, South Korea, Fibroblasts, Gene Therapy, Bioengineering, Connective Tissue Cells
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