By a News Reporter-Staff News Editor at Biotech Week -- Data detailed on Enzymes and Coenzymes have been presented. According to news reporting from Pullman, Washington, by NewsRx journalists, research stated, "Podophyllum species are sources of (-)-podophyllotoxin, an aryltetralin lignan used for semi-synthesis of various powerful and extensively employed cancer-treating drugs. Its biosynthetic pathway, however, remains largely unknown, with the last unequivocally demonstrated intermediate being (-)-matairesinol."
The news correspondents obtained a quote from the research from Washington State University, "Herein, massively parallel sequencing of Podophyllum hexandrum and Podophyllum peltatum transcriptomes and subsequent bioinformatics analyses of the corresponding assemblies were carried out. Validation of the assembly process was first achieved through confirmation of assembled sequences with those of various genes previously established as involved in podophyllotoxin biosynthesis as well as other candidate biosynthetic pathway genes. This contribution describes characterization of two of the latter, namely the cytochrome P450s, CYP719A23 from P. hexandrum and CYP719A24 from P. peltatum. Both enzymes were capable of converting (-)-matairesinol into (-)-pluviatolide by catalyzing methylenedioxy bridge formation and did not act on other possible substrates tested. Interestingly, the enzymes described herein were highly similar to methylenedioxy bridge-forming enzymes from alkaloid biosynthesis, whereas candidates more similar to lignan biosynthetic enzymes were catalytically inactive with the substrates employed."
According to the news reporters, the research concluded: "This overall strategy has thus enabled facile further identification of enzymes putatively involved in (-)-podophyllotoxin biosynthesis and underscores the deductive power of next generation sequencing and bioinformatics to probe and deduce medicinal plant biosynthetic pathways."
For more information on this research see: Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis. Journal of Biological Chemistry, 2013;288(1):466-79. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Journal of Biological Chemistry - www.jbc.org/)
Our news journalists report that additional information may be obtained by contacting J.V. Marques, Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, United States. Additional authors for this research include K.W. Kim, C. Lee, M.A. Costa, G.D. May, J.A. Crow, L.B. Davin and N.G Lewis (see also Enzymes and Coenzymes).
Keywords for this news article include: Antiretrovirals, Drugs, Pullman, Lignans, Therapy, Washington, United States, Podophyllotoxin, Benzene Derivatives, Enzymes and Coenzymes, Tetrahydronaphthalenes, North and Central America.
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