By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Cardiology have been published. According to news reporting from Kitakyushu, Japan, by NewsRx journalists, research stated, "Microwell chip culture is a promising technique for the generation of homogenous embryoid bodies (EBs). In this study, we focused on the relationship between microwell size and mouse EB properties."
The news correspondents obtained a quote from the research from the University of Kitakyushu, "The basic chip design was 195 microwells in a triangular arrangement on a polymethylmethacrylate plate with a surface modified by polyethylene glycol to render it nonadhesive, and 4 similar chips were fabricated with microwell diameters of 400, 600, 800, and 1000 mu m. The cell proliferation rate of EBs in larger microwells was higher than that of EBs in smaller microwells. The decrease in the expression levels of undifferentiated marker genes (Oct3/4 and Nanog) in larger microwells was faster than that in smaller microwells. The expression of hepatic (transthyretin and alpha-fetoprotein), cardiac (Nkx2.5 and alpha-myosin heavy chain), and vascular (fetal liver kinase-1; Flk1) markers in larger microwells was higher than that in smaller microwells. The expression levels of differentiation markers except Flk1 in the chip with a diameter of 1000 mu m were similar to those in hanging drop culture. However, Flk1 expression in microwell chip was markedly lower than that in hanging drop culture, suggesting that microwell chip culture promotes differentiation of hepatic and cardiac lineages. Furthermore, glucose consumption and lactate production were higher in smaller microwells, suggesting that the culture proceeds under anaerobic conditions in smaller microwells."
According to the news reporters, the research concluded: "These results indicate that the difference in microwell size affects the proliferation and differentiation of embryonic stem cells, and that microwell culture is a promising technique to control ER properties."
For more information on this research see: Characterization of mouse embryoid bodies cultured on microwell chips with different well sizes. Journal of Bioscience and Bioengineering, 2013;116(5):628-633. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
Our news journalists report that additional information may be obtained by contacting K. Nakazawa, Univ Kitakyushu, Dept. of Life & Environm Engn, Wakamatsu Ku, Kitakyushu, Fukuoka 8080135, Japan. Additional authors for this research include Y. Yoshiura, H. Koga and Y. Sakai (see also Cardiology).
Keywords for this news article include: Asia, Japan, Kitakyushu, Cardiology
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