By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Gram-Positive Bacteria. According to news reporting from Taejon, South Korea, by NewsRx journalists, research stated, "We describe the development of a new secretory production system for the enhanced production of a single-chain variable fragment (scFv) against the anthrax toxin in Corynebacterium glutamicum. For efficient secretory production of the antibody fragment, the following components were examined: (1) signal peptides, (2) codon usage of antibody fragment, (3) promoters, (4) 5' untranslated region (5' UTR) sequence, and (5) transcriptional terminator."
The news correspondents obtained a quote from the research from the Korea Advanced Institute of Science and Technology (KAIST), "Among all the systems examined, the use of a codon-optimized gene sequence, a Sec-dependent PorB signal peptide, and a fully synthetic H36 promoter, allowed the highest production of antibody fragments in a culture medium. For large-scale production, fed-batch cultivations were also conducted in a 5-L lab-scale bioreactor. When cells were cultivated in semi-defined media, cells could grow up to an OD600 of 179 for 32 h and an antibody fragment concentration as high as 68 mg/L could be obtained in a culture medium with high purity. From the culture medium, the secreted antibody was successfully purified using a simple purification procedure, with correct binding activity confirmed by enzyme-linked immunosorbent assay."
According to the news reporters, the research concluded: "To the best of our knowledge, this is the first report of a fed-batch cultivation for antibody fragment production in C. glutamicum."
For more information on this research see: High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum. Applied Microbiology and Biotechnology, 2014;98(1):273-284. Applied Microbiology and Biotechnology can be contacted at: Springer, 233 Spring St, New York, NY 10013, USA. (Springer - www.springer.com; Applied Microbiology and Biotechnology - www.springerlink.com/content/0175-7598/)
Our news journalists report that additional information may be obtained by contacting S.S. Yim, Korea Adv Inst Sci & Technol, Inst BioCentury, Taejon 305701, South Korea. Additional authors for this research include S.J. An, J.W. Choi, A.J. Ryu and K.J. Jeong (see also Gram-Positive Bacteria).
Keywords for this news article include: Asia, Antibodies, Taejon, Immunology, South Korea, Actinobacteria, Blood Proteins, Actinomycetales, Immunoglobulins, Gram-Positive Rods, Gram-Positive Bacteria, Corynebacterium glutamicum, Irregular Gram-Positive Asporogenous Rods
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