By a News Reporter-Staff News Editor at Biotech Week -- Investigators discuss new findings in Nanoparticles. According to news reporting originating in Tel Aviv, Israel, by NewsRx journalists, research stated, "We present a method for adding molecular specificity to wide-field interferometric phase microscopy (IPM) by recording the phase signatures of gold nanoparticles (AuNPs) labeling targets of interest in biological cells. The AuNPs are excited by time-modulated light at a wavelength corresponding to their absorption spectral peak, evoking a photothermal (PT) effect due to their plasmonic resonance."
The news reporters obtained a quote from the research from Tel Aviv University, "This effect induces a local temperature rise, resulting in local refractive index and phase changes that can be detected optically. Using a wide-field interferometric phase microscope, we acquired an image sequence of the AuNP sample phase profile without requiring lateral scanning, and analyzed the time-dependent profile of the entire field of view using a Fourier analysis, creating a map of the locations of AuNPs in the sample. The system can image a wide-field PT phase signal from a cluster containing down to 16 isolated AuNPs. AuNPs are then conjugated to epidermal growth factor receptor (EGFR) antibodies and inserted to an EGFR-overexpressing cancer cell culture, which is imaged using IPM and verified by confocal microscopy."
According to the news reporters, the research concluded: "To the best of our knowledge, this is the first time wide-field interferometric PT imaging is performed at the subcellular level without the need for total internal reflection effects or scanning."
For more information on this research see: Wide-field interferometric phase microscopy with molecular specificity using plasmonic nanoparticles. Journal of Biomedical Optics, 2013;18(11):133-140. Journal of Biomedical Optics can be contacted at: Spie-Soc Photo-Optical Instrumentation Engineers, 1000 20TH St, PO Box 10, Bellingham, WA 98225, USA (see also Nanoparticles).
Our news correspondents report that additional information may be obtained by contacting N.A. Turko, Tel Aviv University, Fac Engn, Dept. of Biomed Engn, IL-69978 Tel Aviv, Israel. Additional authors for this research include A. Peled and N.T. Shaked.
Keywords for this news article include: Asia, Israel, Tel Aviv, Nanotechnology, Emerging Technologies
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC