By a News Reporter-Staff News Editor at Hematology Week -- Research findings on Lymphoproliferative Disorders are discussed in a new report. According to news reporting originating in Lille, France, by NewsRx journalists, research stated, "With the increasing human exposure to nanoparticles (NP), the evaluation of their genotoxic potential is of significant importance. However, relevance for NP of the routinely used in vitro genotoxicity assays is often questioned, and a nanoparticulate reference positive control would therefore constitute an important step to a better testing of NP, ensuring that test systems are really appropriate."
The news reporters obtained a quote from the research from Institute for Biology Research, "In this study, we investigated the possibility of using tungsten carbide-cobalt (WC-Co) NP as reference positive control in in vitro genotoxicity assays, including 2 regulatory assays, the mouse lymphoma assay and the micronucleus assay, and in the Comet assay, recommended for the toxicological evaluation of nanomedicines by the French Agency of Human Health Products (Afssaps). Through these assays, we were able to study different genetic endpoints in 2 cell types commonly used in regulatory genotoxicity assays: the L5178Y mouse lymphoma cell line and primary cultures of human lymphocytes. Our results showed that the use of WC-Co NP as positive control in in vitro genotoxicity assays was conceivable, but that different parameters have to be considered, such as cell type and treatment schedule. L5178Y mouse lymphoma cells did not provide satisfactory results in the 3 performed tests. However, human lymphocytes were more sensitive to genotoxic effects induced by WC-Co NP, particularly after a 24-h treatment in the in vitro micronucleus assay and after a 4-h treatment in the in vitro Comet assay."
According to the news reporters, the research concluded: "Under such conditions, WC-Co could be used as a nanoparticulate reference positive control in these assays."
For more information on this research see: Tungsten Carbide-Cobalt as a Nanoparticulate Reference Positive Control in In Vitro Genotoxicity Assays. Toxicological Sciences, 2014;137(1):125-134. Toxicological Sciences can be contacted at: Oxford Univ Press, Great Clarendon St, Oxford OX2 6DP, England. (Oxford University Press - www.oup.com/; Toxicological Sciences - toxsci.oxfordjournals.org)
Our news correspondents report that additional information may be obtained by contacting H. Moche, Inst Biol Lille, Plateforme BICeL IFR142, F-59021 Lille, France. Additional authors for this research include D. Chevalier, N. Barois, E. Lorge, N. Claude and F. Nesslany (see also Lymphoproliferative Disorders).
Keywords for this news article include: Lille, France, Europe, Cobalt, Genetics, Lymphoma, Oncology, Chemicals, Chemistry, Treatment, Hematology, Immunology, Blood Cells, Lymphocytes, Tungsten Carbide, Lymphatic Diseases, Transition Elements, Mononuclear Leukocytes, Hemic and Immune Systems, Immunoproliferative Disorders, Lymphoproliferative Disorders
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