By a News Reporter-Staff News Editor at Health & Medicine Week -- Research findings on Proteobacteria are discussed in a new report. According to news reporting from Naples, Italy, by NewsRx journalists, research stated, "We have previously shown that during late stages of the infectious process, serogroup B meningococci (MenB) are able to escape the phagosome of in vitro-infected human epithelial cells. They then multiply in the cytosolic environment and spread intracellularly and to surrounding cells by exploiting the microtubule cytoskeleton, as suggested by results of infections in the presence of microtubule inhibitors and evidence of nanotubes connecting neighboring cells."
The news correspondents obtained a quote from the research from the University of Naples, "In this study, by using microtubule binding assays with purified microtubule asters and bundles and microtubule bundles synthesized in vitro, we demonstrate that the MenB capsule directly mediates the interaction between bacteria and microtubules. The direct interaction between the microtubules and the MenB capsular polysaccharide was confirmed by coimmunoprecipitation experiments. Unexpectedly, serogroup C meningococci (MenC), which have a capsular polysaccharide that differs from that of MenB only by its anomeric linkage, alpha(2 ? 9) instead of alpha(2 ? 8), were not able to interact with the microtubules, and the lack of interaction was not due to capsular polysaccharide O-acetylation that takes place in most MenC strains but not in MenB strains. Moreover, we demonstrate that the MenB capsular polysaccharide inhibits tubulin polymerization in vitro."
According to the news reporters, the research concluded: "Thus, at variance with MenC, MenB may interfere with microtubule dynamics during cell infection."
For more information on this research see: Serogroup-Specific Interaction of Neisseria meningitidis Capsular Polysaccharide with Host Cell Microtubules and Effects on Tubulin Polymerization. Infection and Immunity, 2014;82(1):265-274. Infection and Immunity can be contacted at: Amer Soc Microbiology, 1752 N St NW, Washington, DC 20036-2904, USA. (American Society for Microbiology - www.asm.org; Infection and Immunity - iai.asm.org)
Our news journalists report that additional information may be obtained by contacting A. Tala, University of Naples, Dipartimento Med Sperimentale, Naples, Italy. Additional authors for this research include L. Cogli, M. De Stefano, M. Cammarota, M.R. Spinosa, C. Bucci and P. Alifano (see also Proteobacteria).
Keywords for this news article include: Italy, Naples, Europe, Tubulin, Cytoplasm, Cytoskeleton, Neisseriaceae, Betaproteobacteria, Cellular Structures, Intracellular Space, Microtubule Proteins, Nerve Tissue Proteins, Gram-Negative Bacteria, Neisseria meningitidis, Gram-Negative Aerobic Bacteria, Gram-Negative Aerobic Rods and Cocci
Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC