By a News Reporter-Staff News Editor at Biotech Week -- Investigators publish new report on Enzymes and Coenzymes. According to news originating from Fukuoka, Japan, by NewsRx correspondents, research stated, "Tobacco plant was known to be a non-fructan-storing plant. However, we demonstrated that fructo-oligosaccharides (FOSS) were formed in cured tobacco leaf on adding sucrose to the leaf in our previous report (Nagai et al., J. Agric."
Our news journalists obtained a quote from the research from Kyushu University, "Food Chem., 60, 6606-6612, 2012). Also, it was expected from the results obtained in previous study that FOSS were generated by enzymatic reaction in cured tobacco leaf. The purpose of this study is to confirm and understand the mechanisms of above-mentioned FOSs formation. Thus, we tried to purify the enzymes related to the production of FOSs. The enzymes were extracted from pulverized cured tobacco leaf (burley type leaf), and were purified by charcoal treatment, ultrafiltration, and several chromatography techniques. As a result, one of the enzymes was purified up to 414-fold. It was revealed that this enzyme was acid invertase exhibiting maximum transfructosylation activity at pH 6.0, 60 degrees C. In addition, general properties of this enzyme were also investigated. The enzyme purified in this study enhanced the ratio of FOSs formation under the condition of high concentrated sucrose."
According to the news editors, the research concluded: "From these results, it was suggested that this enzyme participated in the formation of FOSs in tobacco leaf after curing."
For more information on this research see: Transfructosylation reaction in cured tobacco leaf (Nicotiana tabacum). Journal of Bioscience and Bioengineering, 2013;116(6):666-671. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news correspondents report that additional information may be obtained from A. Nagai, Kyushu University, Grad Sch Bioresource & Bioenvironm Sci, Dept. of Innovat Sci & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan. Additional authors for this research include T. Mine, T. Yamamoto and H. Wariishi (see also Enzymes and Coenzymes).
Keywords for this news article include: Asia, Japan, Fukuoka, Enzymes and Coenzymes
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