News Column

New Enzyme Inhibitors Findings Has Been Reported by Researchers at Kansas State University

January 29, 2014



By a News Reporter-Staff News Editor at Biotech Week -- Researchers detail new data in Biotechnology. According to news reporting originating in Manhattan, Kansas, by NewsRx journalists, research stated, "Noroviruses have a single-stranded, positive sense 7-8kb RNA genome, which encodes a polyprotein precursor processed by a virus-encoded 3C-like cysteine protease (3CLpro) to generate mature non-structural proteins. Because processing of the polyprotein is essential for virus replication, norovirus 3CLpro has been targeted for the discovery of anti-norovirus small molecule therapeutics."

The news reporters obtained a quote from the research from Kansas State University, "Thus, we performed functional, structural and inhibition studies of norovirus 3CLpro with fluorescence resonance energy transfer (FRET) assay, X-ray crystallography, and NMR spectroscopy with a synthetic protease inhibitor. Three 3CLpro from Norwalk virus (NV, genogroup I), MD145 (genogroup II) and murine norovirus-1 (MNV-1, genogroup V) were optimized for a FRET assay, and compared for the inhibitory activities of a synthetic protease inhibitor (GC376). The apo 3D structures of NV 3CLpro determined with X-ray crystallography and NMR spectroscopy were further analyzed. In addition, the binding mode of NV 3CLpro-GC376 was compared with X-ray crystallography and NMR spectroscopy."

According to the news reporters, the research concluded: "The results of this report provide insight into the interaction of NV 3CLpro with substrate/inhibitor for better understanding of the enzyme and antiviral drug development."

For more information on this research see: Structural and inhibitor studies of norovirus 3C-like proteases. Virus Research, 2013;178(2):437-44. (Elsevier - www.elsevier.com; Virus Research - www.elsevier.com/wps/product/cws_home/506054)

Our news correspondents report that additional information may be obtained by contacting D. Takahashi, Dept. of Biochemistry, Kansas State University, 141 Chalmers Hall, Manhattan, KS 66506, United States. Additional authors for this research include Y. Kim, S. Lovell, O. Prakash, W.C. Groutas and K.O Chang (see also Biotechnology).

Keywords for this news article include: Antiretrovirals, Antivirals, Biotechnology, Drugs, Kansas, Therapy, Viruses, Protease, Virology, Manhattan, United States, Enzymes and Coenzymes, North and Central America.

Our reports deliver fact-based news of research and discoveries from around the world. Copyright 2014, NewsRx LLC


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Source: Biotech Week


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