By a News Reporter-Staff News Editor at Biotech Week -- Fresh data on Stem Cell Research are presented in a new report. According to news originating from Nishinomiya, Japan, by NewsRx correspondents, research stated, "Aiming for the clinical application of cartilage regeneration, the xeno-free cultivation method to obtain a scaffold-free cartilage-like disc-shaped cell sheet using mesenchymal stem cells (MSCs) derived from human bone marrow without the shrinkage of the sheet was investigated. MSCs were inoculated into Cell Culture Insert (0.3 cm(2), pore size; 0.4 mu m, pore density; 1.0 x 10(8)/cm(2)) using serum-free chondrogenic differentiation medium containing TGF-beta 3, IGF-1 and dexamethasone or other modified media, and cultured at 37 degrees C in 5% CO2 for 3 weeks."
Our news journalists obtained a quote from the research from Mukogawa Women's University, "Sheet thickness, cartilage specific genes expression, ECM accumulation were determined, and the sections of sheets were stained with alcian blue. A novel mixed medium consisting of a growth medium (10% FCS) with a serum-free chondrogenic differentiation medium could prevent the shrinkage of the sheet and produced a disc-shaped cell sheet. The depth of the sheet was approximately 0.7 mm and the gene expression levels were higher than those in cells in normal human cartilage. The use of human serum instead of FCS did not cause shrinkage and did not decrease the accumulation levels of sGAG and type 2 collagen in the sheet. The cultivation of MSCs grown with completely xeno-free materials using the mixed medium containing human serum in a cell culture insert showed a sheet depth of 1.0 mm and gene expression levels higher than those in normal cartilage."
According to the news editors, the research concluded: "The scaffold-free and xeno-free cartilage-like cell sheet was successfully formed without shrinkage using human bone marrow MSCs and the chondrogenic differentiation medium containing human serum."
For more information on this research see: Xeno-free and shrinkage-free preparation of scaffold-free cartilage-like disc-shaped cell sheet using human bone marrow mesenchymal stem cells. Journal of Bioscience and Bioengineering, 2013;116(6):734-739. Journal of Bioscience and Bioengineering can be contacted at: Soc Bioscience Bioengineering Japan, Osaka Univ, Faculty Engineering, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan. (Elsevier - www.elsevier.com; Journal of Bioscience and Bioengineering - www.elsevier.com/wps/product/cws_home/505516)
The news correspondents report that additional information may be obtained from Y. Sato, Mukogawa Womens Univ, Sch Hlth & Sports Sci, Nishinomiya, Hyogo 6638558, Japan. Additional authors for this research include S. Wakitani and M. Takagi (see also Stem Cell Research).
Keywords for this news article include: Asia, Tissue Engineering, Biomedical Engineering, Biomedicine, Japan, Nishinomiya, Bone Marrow, Bone Research, Immune System, Bioengineering, Stem Cell Research, Mesenchymal Stem Cells
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