By a News Reporter-Staff News Editor at Genomics & Genetics Weekly -- A new study on Nervous System Diseases and Conditions is now available. According to news reporting out of New Haven, Connecticut, by NewsRx editors, research stated, "Mutations in Cu,Zn-superoxide dismutase (mtSOD1) cause familial amyotrophic lateral sclerosis (FALS), a neurodegenerative disease resulting from motor neuron degeneration. Here, we demonstrate that wild type SOD1 (wtSOD1) undergoes palmitoylation, a reversible post-translational modification that can regulate protein structure, function, and localization."
Our news journalists obtained a quote from the research from Yale University, "SOD1 palmitoylation was confirmed by multiple techniques, including acyl-biotin exchange, click chemistry, cysteine mutagenesis, and mass spectrometry. Mass spectrometry and cysteine mutagenesis demonstrated that cysteine residue 6 was the primary site of palmitoylation. The palmitoylation of FALS-linked mtSOD1s (A4V and G93A) was significantly increased relative to that of wtSOD1 expressed in HEK cells and a motor neuron cell line. The palmitoylation of FALS-linked mtSOD1s (G93A and G85R) was also increased relative to that of wtSOD1 when assayed from transgenic mouse spinal cords. We found that the level of SOD1 palmitoylation correlated with the level of membrane-associated SOD1, suggesting a role for palmitoylation in targeting SOD1 to membranes. We further observed that palmitoylation occurred predominantly on disulfide-reduced as opposed to disulfide-bonded SOD1, suggesting that immature SOD1 is the primarily palmitoylated species. Increases in SOD1 disulfide bonding and maturation with increased copper chaperone for SOD1 expression caused a decrease in wtSOD1 palmitoylation. Copper chaperone for SOD1 overexpression decreased A4V palmitoylation less than wtSOD1 and had little effect on G93A mtSOD1 palmitoylation."
According to the news editors, the research concluded: "These findings suggest that SOD1 palmitoylation occurs prior to disulfide bonding during SOD1 maturation and that palmitoylation is increased when disulfide bonding is delayed or decreased as observed for several mtSOD1s."
For more information on this research see: Palmitoylation of Superoxide Dismutase 1 (SOD1) Is Increased for Familial Amyotrophic Lateral Sclerosis-linked SOD1 Mutants. Journal of Biological Chemistry, 2013;288(30):21606-21617. Journal of Biological Chemistry can be contacted at: Amer Soc Biochemistry Molecular Biology Inc, 9650 Rockville Pike, Bethesda, MD 20814-3996, USA. (American Society for Biochemistry and Molecular Biology - www.asbmb.org; Journal of Biological Chemistry - www.jbc.org/)
Our news journalists report that additional information may be obtained by contacting S.E. Antinone, Yale University, WM Keck Biotechnol Resource Lab, New Haven, CT 06511, United States. Additional authors for this research include G.D. Ghadge, T.T. Lam, L.J. Wang, R.P. Roos and W.N. Green (see also Nervous System Diseases and Conditions).
Keywords for this news article include: Anions, Cysteine, Genetics, New Haven, Neurology, Disulfides, Connecticut, Electrolytes, United States, Oxidoreductases, Sulfur Amino Acids, Inorganic Chemicals, Neutral Amino Acids, Motor Neuron Disease, Spinal Cord Diseases, Sulfhydryl Compounds, Superoxide Dismutase, Neuromuscular Diseases, TDP-43 Proteinopathies
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