By a News Reporter-Staff News Editor at Biotech Week -- Current study results on Technology have been published. According to news reporting from Ulsan, South Korea, by NewsRx journalists, research stated, "Quantitative real-time PCR (qPCR) has been widely used in recent environmental microbial ecology studies as a tool for detecting and quantifying microorganisms of interest, which aids in better understandings of the complexity of wastewater microbial communities. Although qPCR can be used to provide more specific and accurate quantification than other molecular techniques, it does have limitations that must be considered when applying it in practice."
The news correspondents obtained a quote from the research from the Ulsan National Institute of Science and Technology, "This article reviews the principle of qPCR quantification and its applications to microbial ecology studies in various wastewater treatment environments. Here we also address several limitations of qPCR-based approaches that can affect the validity of quantification data: template nucleic acid quality, nucleic acid extraction efficiency, specificity of group-specific primers and probes, amplification of nonviable DNA, gene copy number variation, and limited number of sequences in the database. Even with such limitations, qPCR is reportedly among the best methods for quantitatively investigating environmental microbial communities. The application of qPCR is and will continue to be increasingly common in studies of wastewater treatment systems."
According to the news reporters, the research concluded: "To obtain reliable analyses, however, the limitations that have often been overlooked must be carefully considered when interpreting the results."
For more information on this research see: Quantitative real-time PCR approaches for microbial community studies in wastewater treatment systems: applications and considerations. Biotechnology Advances, 2013;31(8):1358-73. (Elsevier - www.elsevier.com; Biotechnology Advances - www.elsevier.com/wps/product/cws_home/525455)
Our news journalists report that additional information may be obtained by contacting J. Kim, School of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulsan 689-798, South Korea. Additional authors for this research include J. Lim and C. Lee (see also Technology).
Keywords for this news article include: Asia, Ulsan, Treatment, Technology, South Korea.
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