By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Xi'an, People's Republic of China, by NewsRx journalists, research stated, "Sensitive and specific detection of disease-related gene and single nucleotide polymorphism (SNP) is of great importance in cancer diagnosis. Here, a colorimetric and fluorescent approach is described for detection of the pS3 gene and SNP in homogeneous solution by using gold nanorods (GNRs) as both colorimetric probe and fluorescence quencher."
The news correspondents obtained a quote from the research from Shaanxi Normal University, "Hairpin oligonucleotide was utilized as DNA probe to ensure highly sequence-specific detection of target DNA. In the presence of target DNA, the formation of DNA duplex greatly changed the electrostatic interaction between GNR and DNAs, leading to an obvious change in fluorescence and colorimetric response. The detection limit of fluorescent and colorimetric assay is 0.26 pM and 0.3 nM, respectively. Both fluorescence and colorimetric strategies were able to effectively discriminate complementary DNA from single-base mismatched DNA, which is meaningful for cancer diagnosis. More important, target DNA can be detected as low as 10 nM by the naked eye. Furthermore, transmission electron microscopy and fluorescence anisotropy measurements demonstrated that the color change as well as fluorescence quenching is ascribed to the DNA hybridization-induced aggregation of GNRs."
According to the news reporters, the research concluded: "Therefore, the assay provided a fast, sensitive, cost-effective, and specific sensing platform for detecting disease-related gene and SNP."
For more information on this research see: Gold-Nanorod-Based Colorimetric and Fluorescent Approach for Sensitive and Specific Assay of Disease-Related Gene and Mutation. ACS Applied Materials & Interfaces, 2013;5(22):11741-11746. ACS Applied Materials & Interfaces can be contacted at: Amer Chemical Soc, 1155 16TH St, NW, Washington, DC 20036, USA. (American Chemical Society - www.acs.org; ACS Applied Materials & Interfaces - www.pubs.acs.org/journal/aamick)
Our news journalists report that additional information may be obtained by contacting W.H. Wang, Shaanxi Normal Univ, Key Lab Appl Surface & Colloid Chem, Key Lab Analyt Chem Life Sci Shaanxi Prov, Minist EducSch Chem & Chem Engn, Xian 710062, People's Republic of China. Additional authors for this research include Y.N. Zhao and Y. Jin (see also DNA Research).
Keywords for this news article include: Asia, Xi'an, DNA Research, People's Republic of China
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