By a News Reporter-Staff News Editor at Life Science Weekly -- Current study results on DNA Research have been published. According to news reporting from Alexandria, Egypt, by NewsRx journalists, research stated, "A nanoconjugate was composed of metal oxide nanoparticles decorated with peptides and fluorescent dye and tested for DNA cleavage following UV light activation. The peptide design was based on a DNA binding domain, the so called KH domain of the hnRNPK protein."
The news correspondents obtained a quote from the research from the University of Alexandria, "This 'KH peptide' enabled cellular uptake of nanoconjugates and their entry into cell nuclei. The control nanoconjugate carried no peptide; it consisted only of the metal oxide nanoparticle prepared as Fe3O4@TiO2 nanocomposite and the fluorescent dye alizarin red S. These components of either construct are responsible for nanoconjugate activation by UV light and the resultant production of reactive oxygen species (ROS). Production of ROS at different subcellular locations causes damage to different components of cells: only nanoconjugates inside cell nuclei can be expected to cause DNA cleavage. Degradation of cellular DNA with KH peptide decorated nanoconjugates exceeded the DNA damage obtained from control, no-peptide nanoconjugate counterparts."
According to the news reporters, the research concluded: "Moreover, caspase activation and cell death were more extensive in the same cells."
For more information on this research see: Cytotoxicity and DNA cleavage with core-shell nanocomposites functionalized by a KH domain DNA binding peptide. Nanoscale, 2013;5(23):11394-9. (Royal Society of Chemistry - www.rsc.org/; Nanoscale - pubs.rsc.org/en/journals/journalissues/nr)
Our news journalists report that additional information may be obtained by contacting R. Bazak, Dept. of Otorhinolaryngology and Head & Neck Surgery, University of Alexandria Medical School, Azarita Medical Campus, Champollion Street, Khartoum Square, Alexandria, Egypt. Additional authors for this research include J. Ressl, S. Raha, C. Doty, W. Liu, B. Wanzer, S.A. Salam, S. Elwany, T. Paunesku and G.E Woloschak (see also DNA Research).
Keywords for this news article include: Egypt, Africa, Alexandria, DNA Research, Nanocomposite, Nanoconjugates, Nanotechnology, Emerging Technologies.
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