By a News Reporter-Staff News Editor at Life Science Weekly -- Research findings on Life Science Research are discussed in a new report. According to news reporting from Greensboro, North Carolina, by NewsRx journalists, research stated, "A major problem in the discovery of new biologically active compounds from natural products is the reisolation of known compounds. Such reisolations waste time and resources, distracting chemists from more promising leads."
The news correspondents obtained a quote from the research from the University of North Carolina, "To address this problem, dereplication strategies are needed that enable crude extracts to be screened for the presence of known compounds before isolation efforts are initiated. In a project to identify anticancer drug leads from filamentous fungi, a significant dereplication challenge arises, as the taxonomy of the source materials is rarely known, and, thus, the literature cannot be probed to identify likely known compounds. An ultraperformance liquid chromatography-photodiode array-high-resolution tandem mass spectrometric (UPLC-PDA-HRMS-MS/MS) method was developed for dereplication of fungal secondary metabolites in crude culture extracts. A database was constructed by recording HRMS and MS/MS spectra of fungal metabolites, utilizing both positive-and negative-ionization modes. Additional details, such as UV-absorption maxima and retention times, were also recorded. Small-scale cultures that showed cytotoxic activities were dereplicated before engaging in the scale-up or purification processes. Using these methods, approximately 50% of the cytotoxic extracts could be eliminated from further study after the confident identification of known compounds."
According to the news reporters, the research concluded: "The specific attributes of this dereplication methodology include a focus on bioactive secondary metabolites from fungi, the use of a 10 min chromatographic method, and the inclusion of both HRMS and MS/MS data."
For more information on this research see: High-resolution MS, MS/MS, and UV database of fungal secondary metabolites as a dereplication protocol for bioactive natural products. Journal of Natural Products, 2013;76(9):1709-16. (American Chemical Society - www.acs.org; Journal of Natural Products - www.pubs.acs.org/journal/jnprdf)
Our news journalists report that additional information may be obtained by contacting T. El-Elimat, Dept. of Chemistry and Biochemistry, University of North Carolina at Greensboro , PO Box 26170, Greensboro, North Carolina 27402, United States. Additional authors for this research include M. Figueroa, B.M. Ehrmann, N.B. Cech, C.J. Pearce and N.H Oberlies (see also Life Science Research).
Keywords for this news article include: Greensboro, United States, North Carolina, Life Science Research, North and Central America.
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