By a News Reporter-Staff News Editor at Health & Medicine Week -- Investigators publish new report on Biotechnology. According to news reporting out of Lexington, Kentucky, by NewsRx editors, research stated, "Manipulation of the cellulose biosynthetic machinery in plants has the potential to provide insight into plant growth, morphogenesis and to create modified cellulose for anthropogenic use. Evidence exists that cellulose microfibril structure and its recalcitrance to enzymatic digestion can ameliorated via mis-sense mutation in the primary cell wall-specific gene AtCELLULOSE SYNTHASE (CESA)3."
Our news journalists obtained a quote from the research from the University of Kentucky, "This mis-sense mutation has been identified based on conferring drug resistance to the cellulose inhibitory herbicide isoxaben. To examine whether it would be possible to introduce mutant CESA alleles via a transgenic approach, we overexpressed a modified version of CESA3, AtCESA3(ixr1-2) derived from Arabidopsis thaliana L. Heynh into a different plant family, the Solanceae dicotyledon tobacco (Nicotiana tabacum L. variety Samsun NN). Specifically, a chimeric gene construct of CESA3(ixr1-2) , codon optimized for tobacco, was placed between the heterologous M24 promoter and the rbcSE9 gene terminator. The results demonstrated that the tobacco plants expressing M24-CESA3(ixr1-2) displayed isoxaben resistance, consistent with functionality of the mutated AtCESA3(ixr1-2) in tobacco. Secondly, during enzymatic saccharification, transgenic leaf-and stem-derived cellulose is 54%-66% and 40%-51% more efficient, respectively, compared to the wild type, illustrating translational potential of modified CESA loci."
According to the news editors, the research concluded: "Moreover, the introduction of M24-AtCESA3(ixr1-2) caused aberrant spatial distribution of lignified secondary cell wall tissue and a reduction in the zone occupied by parenchyma cells."
For more information on this research see: Manipulating cellulose biosynthesis by expression of mutant Arabidopsis proM24::CESA3(ixr1-2) gene in transgenic tobacco. Plant Biotechnology Journal, 2013;11(3):362-72. Plant Biotechnology Journal can be contacted at: Blackwell Publishing Inc, 350 Main St, Malden, MA 02148, USA. (Wiley-Blackwell - www.wiley.com/; Plant Biotechnology Journal - onlinelibrary.wiley.com/journal/10.1111/(ISSN)1467-7652)
Our news journalists report that additional information may be obtained by contacting D.K. Sahoo, KTRDC, College of Agriculture, University of Kentucky, Lexington, KY, United States. Additional authors for this research include J. Stork, S. DeBolt and I.B Maiti (see also technology.html">Biotechnology).
Publisher contact information for the Plant Biotechnology Journal is: Blackwell Publishing Inc, 350 Main St, Malden, MA 02148, USA.
Keywords for this news article include: Biotechnology, Kentucky, Lexington, United States, North and Central America.
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