By a News Reporter-Staff News Editor at Gene Therapy Weekly -- Investigators publish new report on Biotechnology. According to news reporting out of San Francisco, California, by NewsRx editors, research stated, "We recently demonstrated that axonal transport of adeno-associated virus (AAV) is serotype-dependent. Thus, AAV serotype 2 (AAV2) is anterogradely transported (e.g., from cell bodies to nerve terminals) in both rat and non-human primate (NHP) brain."
Our news journalists obtained a quote from the research from the University of California, "In contrast, AAV serotype 6 (AAV6) is retrogradely transported from terminals to neuronal cell bodies in the rat brain. However, the directionality of axonal transport of AAV6 in the NHP brain has not been determined. In this study, two Cynomolgus macaques received an infusion of AAV6 harboring green fluorescent protein (GFP) into the striatum (caudate and putamen) by magnetic resonance (MR)-guided convection-enhanced delivery. One month after infusion, immunohistochemical staining of brain sections revealed a striatal GFP expression that corresponded well with MR signal observed during gene delivery. As shown previously in rats, GFP expression was detected throughout the prefrontal, frontal and parietal cortex, as well as the substantia nigra pars compacta and thalamus, indicating retrograde transport of the vector in NHP. AAV6-GFP preferentially transduced neurons, although a few astrocytes were also transduced. Transduction of non-neuronal cells in the brain was associated with the upregulation of the major histocompatibility complex-II and lymphocytic infiltration as previously observed with AAV1 and AAV9. This contrasts with highly specific neuronal transduction in the rat brain."
According to the news editors, the research concluded: "Retrograde axonal transport of AAV6 from a single striatal infusion permits efficient transduction of cortical neurons in significant tissue volumes that otherwise would be difficult to achieve."
For more information on this research see: Adeno-associated virus type 6 is retrogradely transported in the non-human primate brain. Gene Therapy, 2013;20(12):1178-1183. Gene Therapy can be contacted at: Nature Publishing Group, Macmillan Building, 4 Crinan St, London N1 9XW, England. (Nature Publishing Group - www.nature.com/; Gene Therapy - www.nature.com/gt/)
Our news journalists report that additional information may be obtained by contacting W. San Sebastian, University of California, Dept. of Neurol Surg, San Francisco, CA 94103, United States. Additional authors for this research include L. Samaranch, G. Heller, A.P. Kells, J. Bringas, P. Pivirotto, J. Forsayeth and K.S. Bankiewicz (see also Biotechnology).
Keywords for this news article include: Biotechnology, Cells, Neurons, Viruses, Virology, California, Gene Therapy, San Francisco, United States, Bioengineering, Adeno-Associated Virus, North and Central America, Green Fluorescent Protein
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