technology Details Findings in Applied and Environmental Microbiology -->
By a News Reporter-Staff News Editor at Energy Weekly News -- Researchers detail new data in Life Science Research. According to news originating from Stockholm, Sweden, by VerticalNews correspondents, research stated, "Cyanobacteria are emerging as promising hosts for production of advanced biofuels such as n-butanol and alkanes. However, cyanobacteria suffer from the same product inhibition problems as those that plague other microbial biofuel hosts."
Our news journalists obtained a quote from the research from the School of Biotechnology, "High concentrations of butanol severely reduce growth, and even small amounts can negatively affect metabolic processes. An understanding of how cyanobacteria are affected by their biofuel product can enable identification of engineering strategies for improving their tolerance. Here we used transcriptome sequencing (RNA-Seq) to assess the transcriptome response of Synechocystis sp. strain PCC 6803 to two concentrations of exogenous n-butanol. Approximately 80 transcripts were differentially expressed at 40 mg/liter butanol, and 280 transcripts were different at 1 g/liter butanol. Our results suggest a compromised cell membrane, impaired photosynthetic electron transport, and reduced biosynthesis. Accumulation of intracellular reactive oxygen species (ROS) scaled with butanol concentration. Using the physiology and transcriptomics data, we selected several genes for overexpression in an attempt to improve butanol tolerance. We found that overexpression of several proteins, notably, the small heat shock protein HspA, improved tolerance to butanol."
According to the news editors, the research concluded: "Transcriptomics-guided engineering created more solvent-tolerant cyanobacteria strains that could be the foundation for a more productive biofuel host."
For more information on this research see: Using transcriptomics to improve butanol tolerance of Synechocystis sp. strain PCC 6803. Applied and Environmental Microbiology, 2013;79(23):7419-27. (American Society for Microbiology - www.asm.org; Applied and Environmental Microbiology - aem.asm.org)
The news correspondents report that additional information may be obtained from J. Anfelt, School of Biotechnology, KTH-Royal Institute of Technology, Stockholm, Sweden. Additional authors for this research include B. Hallstrom, J. Nielsen, M. Uhlen and E.P Hudson.
Keywords for this news article include: Biotechnology, Sweden, Europe, Energy, Biofuel, Stockholm, Oil and Gas, Bioengineering, Life Science Research.
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